色氨酸羟化酶1和单胺氧化酶A基因对抑郁症患者额叶负性情绪加工功能的叠加效应

目的 利用影像基因组学方法,分析色氨酸羟化酶1基因A218C多态性、单胺氧化酶A基因串联重复序列(u-VNTR)多态性对抑郁症额叶功能的影响,以初步探讨抑郁症额叶功能异常的遗传学基础.方法 对28例抑郁症患者及34例性别、年龄、受教育年限匹配的健康对照进行负性情绪识别功能核磁共振扫描及基因分型,以双侧额叶上、中、下回为感兴趣脑区,比较不同基因型患者组、对照组额叶亚区激活强度的差异及两种多态性之间的交互作用.结果 TPH1 AA基因型患者组(0.19±0.01)、AA基因型对照组(0.15 ±0.05)左额下回激活强度较其余4组显著增强,AA基因型患者组右额下回(0.28±0.07)激活较其余五组显著增强.MAOA高活性基因型患者组右额中回(0.15±0.06)、左额下回(0.18±0.02)激活强度均较低活性基因型患者组、低活性基因型对照组显著增强.两种基因型对左额下回(F=4.98,P=0.047)存在叠加作用,患者组中,AA+H基因型携带者左额下回激活强度显著增强.结论 五羟色胺系统中的不同基因能够通过共同的五羟色胺能作用影响抑郁症大脑功能异常.

Additive effect of tryptophan hydroxylase 1 and Monoamine oxidase A gene on negative emotional management in depressive frontal subregions

Objective To explore the genetic impact of TPH1 A218C,MAOA-uVNTR on abnormal frontal lobe of depressed patients and the interactions between the two polymorphisms using the method of genetic imaging.Methods 28 patients with major depression and 34 healthy controls which were equal in sex,age,years of education and had negative family history of mental illness were recruited in our study.All paticipants underwent functional Magnetic Resonance Imaging (FMRI) in negative emotion recognition and were divided into different genotypes.Then frontal lobe was extracted as region of interest by WFU software into six subregions-bilateral superior frontal lobe,middle frontal lobe and inferior frontal lobe.Results Patients (0.19 ± 0.01 ) and controls (0.15± 0.05 ) with TPH1 AA genotype showed increased activation in left inferior frontal lobe than patients and controls with AC or CC genot.Patients with AA genotype showed increased activation in right inferior frontal gyrus(0.28 ±0.07) than other five groups as well.Patients with MAOA-H genotype showed increased activation in right middle frontal gyrus(0.15 ±0.06),left inferior frontal gyrus(0.18±0.02) than patients and controls with L genotype.Superimposition of TPH1 A218C and MAOA-uVNTR exsited in abnormal function of left inferior frontal gyrus(F=4.98,P =0.047 ).Patients with AA and H genotype showed increased activation in this area significantly than other patient group.Conclusion Different genes in serotonin system can affect brain function through a common 5-HT feature.