| 高速逆流色谱法分离纯化散斑竹根兰中的抗癌活性成分 |
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| 引用本文： | 宋航娜,陈书红.高速逆流色谱法分离纯化散斑竹根兰中的抗癌活性成分[J].河北化工,2009,32(8):45-46. |
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| 作者姓名： | 宋航娜 陈书红 |
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| 作者单位： | 1. 华北制药集团,河北,石家庄,050015
2. 华北制药集团新药研究开发有限责任公司,河北,石家庄,050015 |
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| 摘 要： | 采用高速逆流色谱法（HSCCC）,以正己烷-乙酸乙酯-乙醇-水组成二相系统作为固定相与流动相,对散斑竹根兰的抗癌活性部位进行了分离纯化。结果发现：以正己烷-乙酸乙酯-乙醇-水（体积比为1∶1∶1.1∶0.9）组成的系统可以从散斑竹根兰的抗癌活性部位中分离出其中的抗癌活性成分之一3-（4′-hydroxy-benzyl）-5,7-dihydroxy-6-methyl-8-methoxyl-chroman-4-one,纯度可达95%以上。
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| 关 键 词： | 高速逆流色谱 抗癌活性成分 散斑竹根兰 |
Preparative Isolation and Purification of Anti- cancer Activity Constitute from Disporopsis aspersa (Hua) Engl. ex K. Krause by High-Speed Counter-Current Chromatography |
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| SONG Hang-na,CHEN Shu-hong.Preparative Isolation and Purification of Anti- cancer Activity Constitute from Disporopsis aspersa (Hua) Engl. ex K. Krause by High-Speed Counter-Current Chromatography[J].Hebei Chemical Engineering and Industry,2009,32(8):45-46. |
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| Authors: | SONG Hang-na CHEN Shu-hong |
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| Affiliation: | 1.North China Pharmaceutical Group;Shijiazhuang 050015;China;2.North China Pharmaceutical New Drug Research and Development Co.;Ltd;China |
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| Abstract: | High-speed counter-current chromatography（HSCCC） was used for the separation of anti-cancer activity constitute from Disporopsis aspersa（Hua） Engl.ex K.Krause.Anti-cancer activity constitute was obtained by HSCCC with a binary phase solvent system composed of n-hexane-ethyl acetat-alcohol-water（1∶1∶1.1∶0.9,V/V）.Then,3-（4′-hydroxy-benzyl）-5,7-dihydroxy-6-methyl-8-methoxyl-chroman-4-one was obtained with a system composed of n-hexane-ethyl acetat-alcohol-water（1 ∶1 ∶1.1 ∶0.9,V/V）.High performance liquid chromatographic analysis of 3-（4′-hydroxy-benzyl）-5,7-dihydroxy-6-methyl-8-methoxyl-chroman-4-one revealed that its purities were over 95%. |
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| Keywords: | high-speed counter-current chromatography anti-cancer activity constitute Disporopsis aspersa（Hua） Engl ex K Krause |
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