柚皮苷对人卵巢癌SKOV3细胞环氧化酶2mRNA及蛋白表达水平的影响

目的：研究中药柚皮苷对人卵巢癌SK—OV3细胞环氧化酶2（CoX-2）tuRNA及蛋白表达水平的影响。方法：常规培养人卵巢癌sK—OV3细胞,分为空白对照组、柚皮苷10umol／L组、柚皮苷20tzmol／L组、柚皮苷40umol／L组、阳性对照组（塞来昔布80tLmol／L）。MTT法测定柚皮苷对人卵巢癌SKOV3细胞增殖的影响,运用Real—timePCR和WesternBlot法测定培养48h后各组细胞中COX_2mRNA和蛋白表达水平的变化。结果：MTT检测法显示,柚皮苷各浓度处理组,时间依赖性和剂量依赖性地抑制人卵巢癌SKOV3细胞生长。经药物处理48h后,Real-timePCR结果显示,与空白对照组（1．094±0．053）比较,10、20umol／L柚皮苷即对SK—OV3细胞COX_2mRNA表达抑制作用（O．828±0．006,0．753±0．011,P〈0．05）,而40gmol／L柚皮苷则明显下调COX-2mRNA的表达（0．412±0．216,P〈0．01）,与塞来昔布组（0．321±0．017）的抑制程度相近。WesternBlot法检测结果显示,与空白对照组（1．7325±0．0826）相比,10umol／L柚皮苷组相对表达量（1．7925±0．0880）虽略有上调,但无统计学差异,20、40／umol／L柚皮苷组均可明显下调SKOV3细胞COX-2蛋白的表达（1．2225±0．0822,1．2725±0．0763,P〈0．05）,塞来昔布组也明显抑制CoX-2蛋白的表达。结论：柚皮苷可抑制人卵巢癌SKOV3细胞体外增殖并抑制COX-2mRNA和蛋白的表达。

Effects of Naringin on expression of COX-2 mRNA and protein in Human ovarian cancer cell line SKOV3

AIM： To investigate effects of na- ringin on expression of COX-2 mRNA and pro- tein of Human ovarian cancer cell line SKOV3. METHODS： SKOV3 cells were cultured in vitro and divided into five groups randomly, namelycontrol group, naringin group （10 /umol/L）, na- ringin group （20 Ixmol/L）, naringin group （40 umol/L） and celecoxib group （80/umol/L）. The inhibition effects of naringin on cell- prolifera- tion were determined hy MTT method. The ex-pression of COX-2 mRNA and protein were eval- uated by Western Blot and Real-time quantita- tive PCR after forty-eight hours. RESULTS：Na- ringin could inhibit the growth of SKOV3 cells, and with the increase of the dosage and time, the tumor growth inhibition rate increased signifi- cantly （ P 〈 0.01 ）. Compared with control group, the expression of COX-2 mRNA was down--regulated significantly by every dosage of naringin and celecoxib （0. 828±0. 006, 0. 753± 0. 011, while, 0.4124-0.216, 0.321±0.017）. Mean the expression of COX-2 protein wasdown--regulated significantly by every dosage of naringin and celecoxib （1. 7925±0. 088, 1. 2225 ± 0.0822, 1.2725 ± 0.07632, 0.3075 ± 0.0977）. CONCLUSION： The appropriate dos- age of naringin can inhibit SKOV3 cells growth in vitro, and can down-regulate the expression of COX-2 mRNA and protein.