miR-106b-5p靶向调控细胞周期蛋白D1抑制结直肠癌细胞增殖、迁移与侵袭

目的:探讨miR-106b-5p对结直肠癌(colorectal cancer,CRC)细胞增殖、迁移与侵袭的影响以及其作用机制.方法:实时荧光定量PCR检测miR-106b-5p在CRC组织与相应的癌旁组织、永生化的肠上皮细胞以及肠癌细胞中的表达量;CCK8实验检测DLD1细胞增殖能力;细胞划痕实验检测DLD1细胞的...

miR-106b-5p inhibits proliferation,migration and invasion of colorectal cancer cells via targeting cyclin D1

Objective:To investigate the effect of miR-106b-5p on the proliferation,migration and invasion of colorectal cancer(CRC)cells and its underlying mechanism.Methods:The expression levels of miR-106b-5p in CRC tissues and adjacent tissues,immortalized intestinal epithelial cells and CRC cells were detected by qPCR.CCK8 assay,scratch assay and Transwell assay were used to detect the proliferation,migration and invasion ability of DLD1 cells.The downstream target gene of miR-106b-5p was searched by bioinformatics method.Dual luciferase assay and RNA binding protein immunoprecipitation assay was conducted to determine the relationship between miR-106b-5p and cyclin D1(CCND1).The level of CCND1 protein in cells and tissues was detected by Western blotting assay.Results:Compared with adjacent tissues,the expression level of miR-106b-5p in colorectal cancer tissues was decreased(P<0.05).The expression level of miR-106b-5p in colorectal cancer cells was decreased compared with that in immortalized intestinal epithelial cells(P<0.05).CCK8 assay,scratch assay and Transwell assay showed that overexpression of miR-106b-5p significantly inhibited the proliferation,migration and invasion in DLD1 cells(P<0.05).Starbase database predicts that the downstream target gene of miR-106b-5p was CCND1,and miR-106b-5p acted on the 3'-UTR region of CCND1.Dual luciferase assay and RNA binding protein immunoprecipitation assay confirmed the relationship between miR-106b-5p and CCND1.CCND1 was highly expressed in colorectal cancer tissues and cells(P<0.05),and the overexpression of miR-106b-5p significantly inhibited CCND1 expression in DLD1 cells(P<0.05).Conclusion:Decreased level of miR-106b-5p can inhibits proliferation,migration and invasion of colorectal cancer cells via targeting CCND1.