基于感官差异的会泽旱烟和师宗旱烟香气成分的HS-SPME-GC/MS对比分析研究

传统卷烟感官质量评价结果显示,添加会泽旱烟的卷烟味道要比师宗旱烟更柔和一些,评分也略高。为分析会泽旱烟和师宗旱烟烟叶中挥发性香气成分的差异,通过对萃取头材料、样品量、萃取时间、萃取温度等条件的筛选,选取最优条件,采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC/MS)分析会泽旱烟和师宗旱烟的烟叶样品,结果表明：（1）通过筛选,最优条件为称取0.2 g烟叶样品,使用PDMS/DVB涂层材料的粉色萃取头,在80℃的温度下萃取20 min,然后进行HS-SPME-GC/MS分析。（2）曲靖旱烟所含挥发性成分为66种,师宗旱烟所含挥发性成分为62种。（3）会泽旱烟和师宗旱烟烟叶样品中,挥发性成分最高的均为烟碱与新植二烯,除这2种物质外,挥发性香气成分中含量较高的为碱类和酮类物质。（4）会泽旱烟中的醇类、酸类挥发性成分含量高于师宗旱烟,香气成分比师宗旱烟更均衡一些。固相微萃取的结果与旱烟感官评价的结论基本一致,可为深入研究旱烟品质及风格特征提供理论依据。

Comparative Analysis of Aroma Components of Huize and Shizong Air-cured Tobacco Leaves by HS-SPME-GC/MS Based on Sensory Differences

The sensory quality evaluation results show that the cigarettes with Huize air-cured tobacco leaves have a softer taste than that with Shizong air-cured tobacco leaves, and the score of Huize air-cured tobacco leaves is slightly higher. To analyze the difference of volatile aroma components in Huize and Shizong air-cured tobacco leaves, the optimal conditions were determined through the selection of extraction head material, extraction time, sample amount, extraction temperature and other conditions, then, headspace solid phase microextraction gas chromatography/mass spectrometry (HS-SPME-GC/MS) was used to analyze the samples. The results show that: (1) through screening, the optimal condition is taking 0.2 g tobacco leaf samples, using pink extraction head of PDMS/DVB coating material, extracting them at 80℃ for 20 min, and then conducting HS-SPME-GC/MS analysis; (2) there are 66 volatile components in Huize air-cured tobacco leaves, and 62 volatile components in Shizong air-cured tobacco leaves; (3) among the 2 kinds of tobacco leaf samples, nicotine and neophyte-diene have the highest mass fraction of volatile components; in addition to these two substances, alkali and ketone substances have higher content in the volatile substances; (4) the contents of alcohol and acid volatile components in Huize air-cured tobacco leaves are higher than that of Shizong air-cured tobacco leaves, and the aroma components are more balanced in Huize than that in Shizong air-cured tobacco leaves. In short, the results of solid-phase microextraction are basically consistent with the conclusions of the sensory evaluation of air-cured tobacco leaves, and the study could provide a theoretical basis for in-depth research on the quality and style characteristics of air-cured tobacco leaves.