犬瘟热患犬中性粒细胞cAMP PDE活性变化及中药作用研究

研究犬瘟热患犬中性粒细胞(PMN)中环磷酸腺苷-磷酸二酯酶（cAMP-PDE）的活性变化及中药对其活性的体外调节作用，为抗炎药研究和病毒性炎症的中药治疗积累资料。提取犬瘟热患犬（n=9）和正常犬(n=6)的中性粒细胞制备cAMP-PDE样品，通过高效液相色谱(HPLC)法测定其活性及中药对其活性的影响。结果显示，犬瘟热患犬组中性粒细〖JP2〗胞cAMP-PDE活性（18.55%±2.66%）显著（P<0.05）高于正常组（11.54%±5.16%）。解表药等5类114味中药中芦根（77.27%）、胡黄连（70.41%）、女贞子（65.50%）、香薷（65.16%）、贝母（61.43%）、黄芩（60.67%）、淫羊藿（60.34%）、黄芪（59.13%）、青蒿（56.93%）、秦皮（55.55%）、辛夷（55.03%）、甘草（54.67%）、牛蒡子（53.40%）、麻黄（53.32%）14味对cAMP-PDE活性具有明显抑制作用。结果表明，中性粒细胞cAMP-PDE活性在犬瘟热患犬炎症过程中具有重要作用，对cAMP-PDE活性有明显抑制作用的中药对其炎症可能具有疗效，同时，中药抑制中性粒细胞中cAMP-PDE活性可能是其抗炎作用机理之一。

Activity Change of cAMP Phosphodiesterase in Polymorphonuclear Neutrophils of Dogs with Canine Distemper and Studies on the Effects of Chinese Herbs

The objective of this paper war to study the activity change of cyclic adenosine monophosphate phosphodiesterase (cAMP-PDE) in neutrophils of dogs with canine distemper and its regulation by Chinese herbs in vitro and to provide data for further studies of anti inflammatory agents and Chinese herbal therapies on viral inflammation. cAMP-PDE was obtained from neutrophils which isolated from dogs having distemper (n=9) (distemper group) and normal dogs (n=6) (control group), and the influences of Chinese herbs on activity of the cAMP-PDE was detected by high performance liquid chromatography (HPLC). The results showed that the activity of cAMP-PDE extracted from neutrophils of distemper group (18.55%±2.66%) was significantly higher than that of the control group (11.54%±5.16%) (P<0.05). 14 traditional Chinese medicines out of 5 types 114 medicines (drugs for relieving exterior syndrome etc.) were significantly inhibit the activity of cAMP-PDE ,including rhizoma phragmitis(77.27%), figwortflower picrorhiza rhizome (70.41%), glossy privet fruit (65.50%), haichow elsholtzia herb (65.16%), fritillariae tuber (61.43%), scutellaria (60.67%), sagittate epimedium (60.34%), astragalus mongholicus (59.13%), herba artemisiae annuae (56.93%) cortex fraxini (55.55%), biond magnolia flower (55.03%), glycyrrhiza uralensis (54.67%), fructus Arctii (53.40%) and ephedra herb (53.32%)，respectively.Those results indicate that the activity of cAMP-PDE in neutrophils plays an important role in the progress of inflammation of dogs with canine distemper.The Chinese herbs might have therapeutic effect on canine distemper because of its comparatively strong inhibitory effects on cAMP-PDE,and inhibiting the activity of cAMP-PDE in neutrophils might be one of the mechanism of Chinese herbs anti inflammatory. 