小鼠氧诱导视网膜新生血管形成中促红细胞生成素的变化

目的:观察C57BL/6小鼠氧诱导视网膜新生血管病变模型(oxygen-induced retinopathy,OIR)眼内促红细胞生成素(erythropoietin,EPO)含量的变化。方法:新生C57BL/6小鼠20只,随机分为实验组(n=10)和对照组(n=10)。实验组小鼠于出生后7d(P7)与母鼠共置于密闭的氧箱,含氧75±5mL/L,共培养5d(P5)后回到正常氧环境,含氧20±1mL/L,建立OIR模型。对照组小鼠不进入氧箱,与母鼠一同饲养于正常氧环境。于P17处死各组小鼠,摘取右眼眼球制作组织切片,HE染色后行病理组织学检查,计数突破内界膜视网膜新生血管(retinal neovascularization,RNV)内皮细胞核数。采用放射免疫法测定实验组和对照组小鼠左眼视网膜组织EPO含量。结果:实验组小鼠视网膜突破内界膜RNV内皮细胞核计数为80.0±6.2个,而对照组仅为1.0±0.9个,两者差异有统计学意义(P<0.01)。实验组视网膜EPO含量为80.8±20.7U/L,对照组为14.4±6.8U/L,两者差异有统计学意义(P<0.01),且视网膜组织EPO表达与RNV增生程度呈明显正相关(r=0.58,P<0.01)。结论:小鼠视网膜新生血管形成与眼球局部EPO上调有关。

Expression of erythropoietin in oxygen-induced retinopathy in mouse

·AIM:To evaluate the erythropoietin(EPO)expression in the eye of murine model of oxygen-induced retinopathy(OIR).·METHODS:Totally 20 neonates of C57BL/6 mouse were randomly divided into experiment group(n=10)and control group(n=10),for establishing the OIR model,the pups in the experiment group were exposed to 75±5mL/L oxygen since their postnatal day 7(P7)for 5 days and then to room air(pO2=20±1mL/L)with their nursing dams.The neonates in the control group were kept in the room air condition.All of the pups were sacrificed at P17,their right eyes were made into pathological sections for observing and counting the number of vascular cell nuclei on the vitreal side of the internal limiting membrane.And radioimmunoassay was used to compare the EPO content of the retina tissue in the left eye of the pups in the experiment group and the control group.·RESULTS:The histopathological sections of retina showed the retinal neovascularization(RNV)endothelial nuclei were 80.0±6.2 in the neonates after induced by oxygen and 1.0±0.9 in the neonates growing up in normal air.There was significant difference between those two groups(P<0.01).EPO radioimmunoassay indicated that the EPO content in experiment group was 80.8±20.7U/L,but 14.4±6.8U/L in the control group,it showed significant difference(P<0.01).A significant correlation between EPO content and neovascular endothelial nuclei was noted in the experiment group and control group(r=0.58,P<0.01).·CONCLUSION:The RNV can be successfully established by the murine model of OIR.RNV was associated with the EPO high expression of the eye.·