| 免疫胶体金标记电镜技术在研究埃希氏大肠杆菌987P粘着素抗原结构上的应用 |
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| 引用本文： | 高锦梁,秦爱建,董国雄,袁莉民,邵天浩.免疫胶体金标记电镜技术在研究埃希氏大肠杆菌987P粘着素抗原结构上的应用[J].电子显微学报,1988(4). |
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| 作者姓名： | 高锦梁 秦爱建 董国雄 袁莉民 邵天浩 |
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| 作者单位： | 江苏农学院 扬州 |
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| 摘 要： | 作者以单克隆抗体为第一抗体,应用免疫胶体金标记电镜技术研究了埃希氏大肠杆菌987P粘着素抗原结构。结果表明,该粘着素由相同的亚单位组成,并呈螺旋状结构。每个亚单位包含着几个相同的抗原决定簇分子,与酶联免疫吸附电镜技术相比较,该法除具有单克隆抗体极高特异性的特点外,还其有大小一致的胶体金颗粒致密附着在抗原相关位点上,清晰易于识别的优点。
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| 关 键 词： | 胶体金 大肠杆菌粘着素 987P 结构 |
The Application of Immunogold Staining (IGS) to the Study of the Structucture of 987P Adhesin of Escherichia Coli |
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| Gao Jingliang Qin AiJian Dong Guoxiong Yuan Liming Shao Tianhao Jiangsu Agricultural College.The Application of Immunogold Staining (IGS) to the Study of the Structucture of 987P Adhesin of Escherichia Coli[J].Journal of Chinese Electron Microscopy Society,1988(4). |
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| Authors: | Gao Jingliang Qin AiJian Dong Guoxiong Yuan Liming Shao Tianhao Jiangsu Agricultural College |
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| Affiliation: | Gao Jingliang Qin AiJian Dong Guoxiong Yuan Liming Shao Tianhao Jiangsu Agricultural College |
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| Abstract: | The structure of the 987 P pilus adhesin of E. coli was investigated with monoclonal anti-body EPN2 directed against comformation-specific antigenic determinants by immunogold sta(?)-ing (IGS) and enzyme-linked immuno-sorbent assay (ELISA). IGS had some advantage overELISA. It facilitated more visuable observation and more precision localization of the pilus struc-ture under EM. The results showed that it was composed of a mumber of subunits, eachof which resides at least a few identical or similar antigenic determinants in a quarternarystructural comformation. |
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| Keywords: | immunogold E coil adhesin 987P structure |
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