雪莲果叶总黄酮超声波辅助酶法提取工艺优化及抗氧化活性研究

以雪莲果叶为原料,设计单因素及响应面试验,优化超声波辅助酶法提取雪莲果叶中总黄酮的最佳工艺;以DPPH自由基的清除率及总还原力为指标,评估雪莲果叶总黄酮提取物的抗氧化能力。结果表明:利用超声波辅助酶法提取雪莲果叶总黄酮的最佳工艺为乙醇体积分数30%、料液比(m雪莲果叶∶V乙醇)1∶60(g/mL)、超声温度50℃、超声处理12 min、酶解pH 4.8、纤维素酶质量浓度0.40 mg/mL、酶解时间66 min,此工艺下总黄酮提取率为6.317%。所提取雪莲果叶总黄酮具有抗氧化能力,与浓度呈正相关;在质量浓度为0.6 mg/mL时,DPPH自由基清除率达到71.09%,具有接近维生素C的总还原力。利用超声波辅助酶法提取的雪莲果叶中总黄酮提取物具有较好的抗氧化能力。

Ultrasonic enzymatic extraction of total flavonoids from Saussurea involucrata leaves and its antioxidant capacity analysis

Optimization of ultrasonic enzymatic extraction of total flavonoids from Saussurea involucrata leaves was studied by single factor and response surface experiments.The antioxidant capacity of the total flavonoids extracted from the leaves of S.involucrata was evaluated using the scavenging rate and total reducing power of DPPH·.The results showed that the best technology of total flavonoids from S.involucrata leaves by ultrasonic enzyme method was:adding the crushed leaves of S.involucrata to 30%ethanol solution,with the material liquid ratio 1∶60(g/mL)and the ultrasonic treatment at 50℃,for 12 min.After adjusting the pH to 4.8,with the enzymatic hydrolysis for 66 min under the condition of cellulase concentration 0.40 mg/mL,the yield of total flavonoids was 6.317%under the technology.The total flavonoids extracted from the leaves of S.involucrata had antioxidant capacity,which was positively correlated with the concentration.DPPH·clearance rate reached 71.09%at 0.6 mg/mL,and the total reduction force closed to V C.In conclusion,the extraction of total flavonoids from the S.involucrata leaves by ultrasonic wave enzyme method was feasible and effective,and the extraction rate was high.The total flavonoids extract had better antioxidant capacity.