| RP-HPLC法测定肠尔舒胶囊中盐酸小檗碱的含量 |
| |
| 引用本文： | 汪明志,王建春,张咏梅.RP-HPLC法测定肠尔舒胶囊中盐酸小檗碱的含量[J].西北药学杂志,2012,27(4):312-313. |
| |
| 作者姓名： | 汪明志 王建春 张咏梅 |
| |
| 作者单位： | 1. 南京中医药大学药学院,南京,210046
2. 张家港市中医医院,张家港,215600 |
| |
| 摘 要： | 目的建立肠尔舒胶囊中盐酸小檗碱含量测定的方法。方法采用RP-HPLC法。色谱柱Sun Fire C18(250mm×4.6mm,5μm);流动相:乙腈-0.05mol·L-1磷酸二氢钾(磷酸调pH为3.0)(25∶75);检查波长:264nm;流速:1.0mL·min-1;柱温:30℃。结果盐酸小檗碱进样量在0.114～1.14μg范围内线性关系良好,r为0.999 9,平均回收率为97.9%(n=9),RSD为1.3%。结论该法准确、简便、快速,可用于肠尔舒胶囊中盐酸小檗碱的含量测定。
|
| 关 键 词： | 肠尔舒胶囊 盐酸小檗碱 高效液相色谱法 |
Determination of berberine hydrochloride in Changershu Capsules by RP-HPLC |
| |
| WANG Mingzhi
,
WANG Jianchun
,
ZHANG Yongmei.Determination of berberine hydrochloride in Changershu Capsules by RP-HPLC[J].Northwest Pharmaceutical Journal,2012,27(4):312-313. |
| |
| Authors: | WANG Mingzhi WANG Jianchun ZHANG Yongmei |
| |
| Affiliation: | 1.College of Pharmacy,Nanjing University of TCM,Nanjing 210046,China;2.Zhangjiagang City Hospital of Chinese Medicine,Zhangjiagang 215600,China) |
| |
| Abstract: | Objective To develop a method for determining berberine in Changershu Capsules.Methods RP-HPLC was used for the determination berberine hydrochloride in Changershu Capsules.The column was Sun Fire C18(250 mm×4.6 mm,5 μm);the mobile phase was acetonitrile-0.05 mol·L-1 KH2PO4(pH3.0)(25∶75);the detection wavelength was 264 nm;the flow rate was 1.0 mL·min-1,and the temperature was at 30 ℃.Results The berberine hydrochloride showed a good linear relationship within 0.114-1.14 μg(r=0.999 9),and the average recovery was 97.9% with RSD 1.3%(n=9).Conclusion This method was accurate,simple,reliable,and can be used for the determination of berberine hydrochloride in Changerchu Capsules. |
| |
| Keywords: | Changershu Capsules berberine hydrochloride RP-HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
