大鼠去卵巢4周和12周骨代谢的变化

目的　动态观察大鼠去卵巢 4周和 12周时骨代谢情况。方法　将 2 8只 4月龄SD雌性大鼠随机分为 4组 :①假手术 4周组 ( 5月龄 )、②去卵巢 4周组、③假手术 12周组 ( 7月龄 )和④去卵巢 12周组。通过骨组织形态计量学方法分析大鼠骨代谢变化情况。结果　去卵巢 4周组与假手术 4周组比 ,以及去卵巢 12周组与假手术 12周组比 ,骨小梁面积百分率 ( %Tb .Ar)和骨小梁数量 (Tb .N)都显著下降 ,同时所有反映骨形成和骨吸收的参数都增加 ;去卵巢 12周组与去卵巢 4周组比 ,%Tb .Ar无显著性差异。结论　大鼠去卵巢后不论 4周还是 12周 ,骨形成和骨吸收活跃程度都增加 ,骨量都显著降低。大鼠去卵巢后前 4周即出现显著的骨丢失 ,以后丢失速度减慢。 7月龄大鼠与 5月龄大鼠比 ,无论去卵巢与否 ,其骨形成和骨吸收活性都降低。

Changes of Bone Metabolism in Rats of 4 and 12 Week after Ovariectomized

ObjectiveTo observe changes of bone metabolism in rats of 4 and 12 week after ovariectomized (OVX).Methods 28 female Sprege Dawley rats (4 month age)were randomly divided into four groups:①Sham OVX 4 weeks group(5 month age);②OVX 4 week group;③Sham OVX 12 weeks group(7month age);④OVX 12 weeks group. The changes of bone metabolism with quantitative bone histomophometry were observed. Results\ In comparison with sham OVX 4 weeks group, the OVX 4 weeks group's Trabecular area percent (%Tb.Ar) decreased, and all the parameters that reflect bone resorption and formation increased. In comparison with Sham OVX 12 weeks group, the parameter of OVX 12 weeks group show same change with OVX 4 weeks group compared with Sham OVX 4 weeks group. %Tb.Ar of OVX 12 weeks group didn't show significant change compared with OVX 4 weeks group.Conclusion\ ①The activity of bone formation and resorption increased and bone volume decreased in OVX 4 and 12 weeks rats.②OVX 4 week group,the speed of bone lost is most fast in the first 4 weeks. ③Compared with 5 month age rats, the activity of bone formation and resorption of 7 monthp age rats decreased, regardless of sham OVX or OVX group.