云南丽江山慈菇遗传多样性的DALP分析

采用DALP (Direct amplification of length polymorphism) 分子标记技术, 对产自云南的药用植物丽江山慈菇Iphigenia indica (L.) Kunth的9个居群进行DNA指纹检测。筛选出5个引物组合, 扩增共产生131条DNA片段, 其中104 条谱带具有遗传多态性, 约占79 39%, 平均每组引物扩增所得多态条带为20 8, 9个居群平均多态百分率为42 21%。9个居群平均观察等位基因数Na为1 4224, 总Na为1 7939; 平均有效等位基因数Ne 为1 3141, 总Ne 为1 4810; 平均遗传多样性指数H为0 1745, 总H为0 2831; 平均Shannon 多样性指数I 为0 2527, 总I为0 4231; 总基因多样性Ht为0 2831, 居群内多样性Hs 为0 1745, 居群间基因分化系数Gst为0 3834, 即丽江山慈菇有61 66%的遗传变异来自居群内, 38 34%来自居群间, 居群间存在较高水平的遗传分化。滇西北居群的遗传多样性明显高于滇中居群的遗传多样性, 这与滇中地区丽江山慈菇野生资源被大规模挖掘有着直接的关系。

DALP Analysis on Genetic Diversity of Iphigenia indica in Yunnan

DALP(Direct amplification of length polymorphism) was applied to detect DNA fingerprints of 9 populations of Iphigenia indica (L.) Kunth in Yunnan.Five primer groups were screened in the 9 populations.Among 131 DNA fragments amplified 104 were polymorphic,i.e.,the percentage of polymorphic bands (PPB) in the 9 populations of Iphigenia indica was 79.39%.The average number of DNA polymorphic band amplified by each primer group was 20.8 and the average PPB was 42.21%.In the 9 populations,the total observed number of alleles (Na) was 1.7939 and the average Na was 1.4224;the total effective number of alleles (Ne) was 1.4810 and the average Ne was 1.3141;the total of Nei's (1973) gene diversity (H) was 0.2831 and the average H was 0.1745;the total Shannon's Information index (I) was 0.4231 and the average I was 0.2527;the total gene diversity (Ht) was 0.2831 while the gene diversity within a population (Hs) was 0.1745.The coefficient of gene differentiation (Gst) was 0.3834 between populations,namely 38.34% genetic variation occurring between populations and 61.66% within a population.The genetic diversity of population in northwestern Yunnan was evidently higher than that in central Yunnan.This might be a result of overexploitation of the resources of this species in central Yunnan in recent years.