肿瘤坏死因子对溶血卵磷脂引起脑微血管平滑肌细胞增殖的影响及药物的保护作用

目的研究肿瘤坏死因子（ＴＮＦ α）对溶血卵磷脂（ＬＰＣ）引起脑微血管平滑肌细胞（ＳＭＣ）增殖的影响及维生素Ｅ（ＶｉｔＥ），普罗布考（ＰＲＢ）的保护作用。方法用结晶紫染色测定密度（ＯＤ）值的方法观察ＴＮＦ α对ＬＰＣ引起牛脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖作用的影响。结果ＴＮＦ α在２５×１０４～１×１０５Ｕ·Ｌ－１范围内能协同ＬＰＣ引起ＢＣＭＳＭＣ增殖，８ｈ时增殖率就明显增加，１６ｈ增殖率达峰值（６７２％）。ＶｉｔＥ２０，５０，１００μｍｏｌ·Ｌ－１，ＰＲＢ１０，２０，５０μｍｏｌ·Ｌ－１呈剂量依赖性地显著降低其增殖率，其最大抑制率分别为７４２％和４９７％。结论ＴＮＦ α对ＬＰＣ引起的ＢＣＭＳＭＣ增殖有协同作用，药物ＶｉｔＥ，ＰＲＢ可拮抗其增殖作用。

Promotive effects of tumor necrosis factor on proliferation of bovine cerebral microvascular smooth muscle cells inducedby lysophosphatidylcholine and the protection of drugs

AIM To investigate promotive effects of tumor necrosis factor (TNF) on bovine cerebal microvescular smooth muscle cells (BCMSMC) proliferation induced by lysophosphatidylcholine (LPC) and the protection of vitamin E (Vit E), probucol (PRB). METHODS The proliferation of BCMSMC was determined by crystal voilet. RESULTSAt concentration from 25104 UL-1 to 1105 UL-1, the proliferation induced by TNF plus LPC (10 nmolL-1) was obviously higher than that induced by LPC alone in a dosedependent manner. TNF significantly promoted the proliferation rate induced by LPC at 8 h and reached the maxium (672%) at 16 h. Vit E and PRB inhibited strikingly proliferation rate in a dosedependent manner. Their maximal inhibitory effects were 742% and 497% respectively. CONCLUSIONS There was a cooperative effection on promotion BCMSMC proliferation by TNF plus LPC on dosedependent manner. Vit E, PRB may protect the BCMSMC from proliferation.