雷帕霉素对二种鳞翅目昆虫细胞自噬和凋亡的影响

以2种鳞翅目昆虫细胞为材料,采用雷帕霉素进行处理,初步研究自噬作用与昆虫细胞凋亡的关系。结果表明:雷帕霉素能够提高家蚕细胞系BMN-e细胞的自噬水平,并能诱导BMN-e细胞发生凋亡;自噬抑制剂3-甲基腺嘌呤能抑制雷帕霉素诱导的BMN-e细胞凋亡。相反,雷帕霉素虽能诱导斜纹夜蛾细胞系SL-HP细胞的自噬水平提高,但不能诱导斜纹夜蛾Spodoptera litura(Fabricius)细胞发生凋亡;雷帕霉素的预处理能抑制放线菌素D诱导的斜纹夜蛾细胞系SL-HP细胞发生凋亡;自噬抑制剂3-甲基腺嘌呤对放线菌素D诱导的细胞凋亡没有影响。因此家蚕Bombyx mori细胞自噬水平的提高与细胞凋亡具有正相关性,而斜纹夜蛾细胞自噬水平的提高与细胞凋亡不相关,相反还对细胞凋亡的诱导具有一定的抑制作用。

Influence of rapamycin on autophagy and apoptosis of two lepidopteran cell lines

In order to understand the relationship between autophagy and apoptosis, we investigated autophagy and apoptosis in two Lepidopteran cell lines, BMN-e cell, and Spodoptera litura（Fabricius） cell line （SL-HP） after treatment with rapamycin. Rapamycin induced antophagy and apoptosis in BMN-e cells, while 3-methyladenine suppressed autophagy and apoptosis induced by rapamycin in BMN-e ceils. However, rapamycin induced autophagy but did not induce apoptosis in SL-HP cells. When the cells were treated with rapamycin in advance, apoptosis induced by actinomycin D was partially inhibited, but 3-methyladenine had no effect on apoptosis induced by actinomycin D. It indicated that autophagy and apoptosis in BMN-e cells had a positive correlation. In contrast, there was no correlation in SL-HP cells, and the increasing of autophagy inhibited apoptosis in this cell line in some extent.