| 金耳、银耳、木耳多酚提取及其抗氧化活性 |
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| 引用本文： | 陈龙,李文峰,令博,明建.金耳、银耳、木耳多酚提取及其抗氧化活性[J].食品科学,2011,32(20):52. |
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| 作者姓名： | 陈龙 李文峰 令博 明建 |
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| 作者单位： | 西南大学食品科学学院;重庆市特色食品工程技术研究中心; |
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| 基金项目： | 重庆市科技攻关项目(CSTC2011AC1010); 西南大学博士基金项目(SWU109005); 中央高校基本科研业务费专项(XDJK2009C044) |
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| 摘 要： | 以金耳、银耳、木耳为原料,分别用80%丙酮、80%甲醇、乙酸乙酯、石油醚对3种食用菌多酚类物质进行提取。通过高效液相色谱法和体外清除自由基方法(DPPH法、O-2.清除能力和.OH清除能力),对3种食用菌多酚类物质的主要组成及体外抗氧化能力进行初步研究。结果表明,80%丙酮提取效果最好,3种食用菌中槲皮素含量相当,金耳和银耳中芦丁和表儿茶素含量较高,木耳中儿茶素和绿原酸含量较高;体外抗氧化结果显示3种食用菌提取液具有较强的自由基清除能力和Fe3+还原能力,其中对DPPH.与O-2.的清除能力较强,对.OH的清除能力较弱。
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| 关 键 词： | 金耳 银耳 木耳 多酚 抗氧化 |
Extraction and Antioxidant Activity of Polyphenols from Tremella aurantialba,Tremella fucitormis and Auricularia aurricula |
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| CHEN Long,LI Wen-feng,LING Bo,MING Jian.Extraction and Antioxidant Activity of Polyphenols from Tremella aurantialba,Tremella fucitormis and Auricularia aurricula[J].Food Science,2011,32(20):52. |
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| Authors: | CHEN Long LI Wen-feng LING Bo MING Jian |
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| Affiliation: | CHEN Long1,LI Wen-feng1,LING Bo1,MING Jian1,2,(1.College of Food Science,Southwest University,Chongqing 400715,China,2.Chongqing Special Food Programme and Technology Research Center,China) |
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| Abstract: | The polyphenols in Tremella aurantialba,Tremella fucitormis and Auricularia aurricula were extracted with 80% acetone,80% methanol,ethyl acetate and petroleum ether,respectively,and their major components and antioxidant activity were determined using high performance liquid chromatography(HPLC) and in vitro DPPH,hydroxyl and superoxide anion radical scavenging assays,respectively.The results revealed that 80% acetone showed the highest extraction efficiency of ployphenols.Tremella aurantialba,Tremella fuci... |
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| Keywords: | Tremella aurantialba Tremella fucitormis Auricularia aurricula polyphenol antioxidant activity |
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