七氟烷和异氟烷对肝脏缺血再灌注损伤的影响

 【目的】探讨七氟烷和异氟烷预处理对大鼠肝脏缺血再灌注损伤(I/R)的保护作用及机制。【方法】96只SD大鼠随机分为假手术对照组(Sham)、肝脏缺血再灌注组(IR)、七氟烷预处理(Sev)和异氟烷预处理组(Iso),于肝脏缺血30min,再灌注30min、120min测各组大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)的含量以及肝脏组织中谷胱甘肽(GSH)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。【结果】肝脏缺血再灌注后IR、Sev和Iso组血清ALT、AST和LDH含量均显著升高,肝组织SOD和GSH显著降低,而MDA明显升高,和Sham组相比有显著差异(P<0.01);Sev、Iso组和IR组比较,ALT、AST、LDH和MDA降低,而SOD和GSH显著升高(P<0.05或P<0.01),其中Sev组比Iso组效果更为明显(P<0.05或P<0.01)。【结论】七氟烷和异氟烷对肝脏缺血再灌注损伤有一定的保护作用,这可能与其抑制氧自由基生成,减少脂质过氧化有关。

Effect of Sevoflurane and Isoflurane on Liver against Ischemia-reperfusion Injury in Rats

Objective] To investigate the protective effect of sevoflurane and isoflurane on the liver against ischemia-reperfusion injury and the mechanisms involved. Methods] Ninety-six SD rats were randomly divided into four groups: sham-operation group (Sham), ischemia-reperfusion group (IR), sevoflurane group (Sev) and isoflurane group (Iso). Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were determined after ischemia-reperfusion. Superoxide dismutase (SOD) activities, glutathione (GSH) and malondialdehyde (MDA) content in liver were measured. Result] The activities of serum ALT, AST, and LDH in IR, Sev, and Iso groups were significantly higher than that in Sham group (P < 0.01) after ischemia-reperfusion. Compared with those in Sham group, SOD activities, GSH content in liver degraded and meanwhile the content of MDA increased in IR, Sev, and Iso groups after ischemia-reperfusion (P < 0.05 or P < 0.01). The activities of ALT, AST, LDH and the content of MDA were lower than those in IR group (P < 0.05 or P < 0.01). Meawhile the SOD activities and GSH content in liver were higher than those in IR group (P < 0.05 or P < 0.01). Conclusion] Both sevoflurane and isoflurane can protect liver from ischemia and reperfusion injury. The protective mechanisms maybe related to reduceing the release of oxyradical and inhibiting the lipid peroxidation.