| 十二碳二元酸产生菌热带假丝酵母的诱变 |
| |
| 引用本文： | 孙凤麟,孙玉梅,何连芳,王淑华.十二碳二元酸产生菌热带假丝酵母的诱变[J].中国酿造,2008(21). |
| |
| 作者姓名： | 孙凤麟 孙玉梅 何连芳 王淑华 |
| |
| 作者单位： | 大连工业大学生物与食品工程学院;辽宁大学轻型产业学院; |
| |
| 基金项目： | 大连市科技攻关项目资助(20010618) |
| |
| 摘 要： | 以热带假丝酵母菌(Candida tropicalis)为出发菌株,经紫外线和氯化锂复合诱变或亚硝酸诱变获得突变株。通过摇瓶分批发酵,对菌株诱变前后发酵产长链二元酸量进行了测定。筛选出能够发酵产酸的突变株12株,其中8株表现出正突变,菌株最高产酸量提高了9.16%。
|
| 关 键 词： | 热带假丝酵母菌 十二碳二元酸 诱变 |
Induced mutation of dodecanedioic acid producing Candida tropicalis |
| |
| SUN Fenglin,SUN Yumei,HE Lianfang,WANG Shuhua.Induced mutation of dodecanedioic acid producing Candida tropicalis[J].China Brewing,2008(21). |
| |
| Authors: | SUN Fenglin SUN Yumei HE Lianfang WANG Shuhua |
| |
| Affiliation: | SUN Fenglin1,SUN Yumei1,HE Lianfang1,WANG Shuhua2 (1. College of Bio & Food Technology,Dalian Polytechnic University,Dalian 116034,China,2. College of Light Industry,Liaoning University,Dalian 110036,China) |
| |
| Abstract: | In this study,Candida tropicalis,the dodecanedioic acid producer,was mutated by complex mutation of ultraviolet and lithium chloride or single mutation of nitrous acid. By batch fermentation in shaking flask,the dodecanedioic acid production of C. tropicalis before and after mutation was determined. The results showed that 12 mutated strains could produce dodecanedioic acid,and 8 of them were positive mutants. The highest do-decanedioic acid yield was improved by 9.16%. |
| |
| Keywords: | Candida tropicalis dodecanedioic acid induced mutation |
| 本文献已被 CNKI 等数据库收录！ |
|
