5种天然植物提取物抗氧化性和酪氨酸酶抑制作用的比较

本研究检测了5种天然植物提取物体外抗氧化及酪氨酸酶活性抑制作用,并分析其黄酮和总酚含量,探讨提取物美白与抗氧化之间的关联。研究表明通过ABTS+、DPPH+、羟自由基、超氧阴离子自由基的清除能力测定得5种提取物的抗氧化能力强弱顺序虽有所差异,但四种抗氧化方法均表明提取物具有一定的抗氧化能力。同时酪氨酸酶活性抑制检测也表明它们均对酶活有一定抑制作用。5种植物提取物抗氧化及酪氨酸酶活性抑制能力均随样品浓度增加而增大,这些结果提示提取物抗氧化能力与其对酪氨酸酶活抑制作用相关联,可能是与提取物中黄酮和总酚物质有关。当采用ABTS+、DPPH+方法时,5种提取物的抗氧化性与酪氨酸酶抑制作用相关性更大,这可能与黄酮和酚类物质对酪氨酸酶活性抑制机理有关,研究结果可为天然美白植物原料的筛选和研发提供一定理论依据与借鉴。

Comparison on the Antioxidant Activity and Tyrosinase Inhibitory Effect of Five Natural Plant Extracts

This study examined the in vitro antioxidant activity and tyrosinase inhibitory effect of five natural plant extracts, analyzed the flavonoid and total phenolic contents, and explored the correlation between whitening effect and antioxidant activity of extracts. The results of ABTS+, DPPH+, hydroxyl and superoxide anion radical scavenging assays revealed that the five extracts all possessed some antioxidant activity but their potencies different. At the same time, tyrosinase inhibition assay showed that these extracts all had some inhibitory effects on enzyme activity. The antioxidant activities and tyrosinase y inhibitory capacities of the five plant extracts increased with elevated concentrations of the sample. These results suggest that the antioxidant capacity of the extract is related to its inhibitory effect on tyrosinase activity, which may be associated with flavonoids and total phenolics in the extracts. When ABTS+ and DPPH+ methods were used, the antioxidant activities of the five extracts were related closer to their corresponding tyrosinase inhibitory effects. This finding may be associated with the inhibitory functions of flavonoids and phenolics towards tyrosinase activity. The research results provide a theoretical basis and reference for the screening and development of natural whitening plant-based raw materials.