Caspase-3、Bcl-2与Bax在血管瘤组织中的表达及其意义

目的：探讨半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）、Bcl-2与Bax的表达及其与血管瘤消退的关系。方法：采用免疫组化sP法检测Caspase-3、Bcl-2与Bax在34例血管瘤和6例正常皮肤组织中的表达。结果：Caspase-3、Bcl-2与Bax在34例血管瘤组织中的阳性表达率，增生期分别为31．3％、81．3％、37．5％，消退期分别为72．2％、38．9％、77．8％，而在正常皮肤均不表达，其中，Bcl-2在增生期中表达高于消退期，差异有统计有统计学意义（P〈0．05），Caspase-3及Bax在消退期中的表达高于增生期，差异有统计学意义（P〈0．05）。结论：Caspase-3、Bcl-2与Bax参与了血管瘤由增殖期转变为消退期的病理发生过程，Caspase-3的活化及Bcl-2与Bax的平衡诱导了细胞凋亡的发生，这可能是血管瘤自发消退的主要因素之一。

Expression of Caspase-3,Bcl-2 and Bax in Hemangiomas

Objective To study the relationship between expression of Caspase-3,Bcl-2,Bax and regression of hemangioma. Methods Caspase-3 ,Bcl-2 and Bax expression was detected in 34 hemangiomas and 6 normal skin tissues by immunohisto-chemistry （SP）.Results In 34 hemangiomas,the positive rates of Caspase-3,Bcl-2 and Bax expression in proliferating phase were 31.3%, 81.3%, 37.5%, in involuting phase were72.2%, 38.9% ,77.8% ,otherwise, those were scarcely expressing in normal skin. The expression of bcl-2 in proliferating phase was higher than in involuting phase （P〈0.05）. Oppositly,the expression of caspase-3 and Bax in proliferating phase was lower than in involuting phase （P〈0.05）. Conclusion Caspase-3 is involved in the converting of hemangiomas from proliferating phase to involuting phase.The activation of Caspase-3 and the balance on Bcl-2 and Bax may induce apoptosis of endothelial cells,thus leading to the regression of hemagioma.