| 10%四氯化碳致大鼠慢性肝损伤的研究 |
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| 引用本文： | 王宇,程薇波,张银柱,王文冬.10%四氯化碳致大鼠慢性肝损伤的研究[J].现代预防医学,2006,33(5):701-703. |
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| 作者姓名： | 王宇 程薇波 张银柱 王文冬 |
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| 作者单位： | 四川大学公共卫生学院病理教研室,四川,610021 |
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| 摘 要： | 目的：研究10％四氯化碳致大鼠慢性肝损伤中间病理变化，为建立10％四氯化碳慢性肝损伤模型提供实验依据。方法：雄性Wistar大鼠80只，随机分为正常对照组和实验组。每组动物各40只。正常对照组腹腔注射玉米油，0．1ml／100g．bw；实验组腹腔注射10％四氯化碳，0．1ml／100g．bw。于实验期的第1、3、6和8周，两组动物分别腹腔注射玉米油和四氯化碳后48h放血处死，取肝组织作组织病理学观察。结果：实验组染毒1周后，肝小叶中央静脉周围大量细胞脂肪变性，肝小叶中央区有少量细胞气球样变；实验组染毒3周后，肝小叶中央静脉周围大量细胞脂肪变性，肝小叶中央区有少量细胞气球样变；实验组染毒3周后．肝小叶中央静脉周围大量细胞脂肪变性，肝小叶中央区有少量细胞气球样变；实验组染毒3周后，肝小叶中央静脉周围肝细胞气球样变加重；实验组染毒6周后，肝小叶中央静脉周围开始出现少量细胞坏死；实验组染毒第8周后，出现肝纤维化，未达到肝硬化。结论：建议少用该模型鉴定药物和保健食品对慢性肝损伤的保护作用。
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| 关 键 词： | 四氯化碳 慢性肝损伤 病理变化 |
| 文章编号： | 1003-8507（2006）05-0701-03 |
| 收稿时间： | 2005-04-30 |
| 修稿时间： | 2005年4月30日 |
A STUDY OF CHRONIC INJURY MODEL INDUCED BY 10% CARBON TETRACHLORIDE |
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| WANG Yu, CHENG Wei-Bo, ZHANG Yin-zhu,et al..A STUDY OF CHRONIC INJURY MODEL INDUCED BY 10% CARBON TETRACHLORIDE[J].Modern Preventive Medicine,2006,33(5):701-703. |
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| Authors: | WANG Yu CHENG Wei-Bo ZHANG Yin-zhu |
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| Affiliation: | Department of Pathology, West China School of Public health, Sichuan University, Chengdu 610041 |
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| Abstract: | Objective:To observe pathological changes of liver induced by 10% carbon tetrachloride in rats,and to make an annimal model of it.Methods:Eighty Wistar rats were randomly devided into 2 groups.The samples of liver were taken at 48 hours after poisoning with the hematoxylin-eosin.Masson,Relculin stains and examined under light microscope.Result:The first week,the hepatic cells occurred fatty degeneration and ballooning degeneration;in the sixth week,Necrotic hepatic cells around centric vein were observed;in the eighth week,the liver turned into Hepatic fibrosis.Conclusion:The chronic model in rats induced by 10% CCl_4 should not be use to judge the positive effect of medicine and health food on chronic liver injury. |
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| Keywords: | Carbon tetrachloride Chronic Liver |
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