Nondestructive, Stereological Estimation of Canopy Surface Area |
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Authors: | Dvoralai Wulfsohn Marco Sciortino Jesper M Aaslyng Marta García-Fiñana |
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Affiliation: | Department of Agriculture and Ecology, Faculty of Life Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark;Dipartimento di Scienze e Tecnologie Agromabientali, UniversitàDegli Studi di Bologna—Alma Mater Studorium, 40127 Bologna, Italy;AgroTech. Institute for Agro Technology and Food Innovation, DK-2630 Taastrup, Denmark;Centre for Medical Statistics and Health Evaluation, University of Liverpool, Liverpool L69 3GS, United Kingdom |
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Abstract: | Summary . We describe a stereological procedure to estimate the total leaf surface area of a plant canopy in vivo, and address the problem of how to predict the variance of the corresponding estimator. The procedure involves three nested systematic uniform random sampling stages: (i) selection of plants from a canopy using the smooth fractionator , (ii) sampling of leaves from the selected plants using the fractionator , and (iii) area estimation of the sampled leaves using point counting . We apply this procedure to estimate the total area of a chrysanthemum ( Chrysanthemum morifolium L. ) canopy and evaluate both the time required and the precision of the estimator. Furthermore, we compare the precision of point counting for three different grid intensities with that of several standard leaf area measurement techniques. Results showed that the precision of the plant leaf area estimator based on point counting is high. Using a grid intensity of 1.76 cm2/point we estimated plant and canopy surface areas with accuracies similar to or better than those obtained using image analysis and a commercial leaf area meter. For canopy surface areas of approximately 1 m2 (10 plants), the fractionator leaf approach with sampling fraction equal to 1/9 followed by point counting using a 4.3 cm2/point grid produced a coefficient of error of less than 7%. The smooth fractionator can be used to ensure that the additional contribution to the estimator variance due to between-plant variability is small. |
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Keywords: | Chrysanthemum morifolium L Coefficient of error Fractionator Nested cluster sampling Point counting Smooth fractionator Stereology Surface area Systematic sampling Variance prediction |
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