| 薄层层析-高效液相色谱法测定食品中苏丹红 |
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| 引用本文： | 李春香,黄坚,马慧雪,张祥.薄层层析-高效液相色谱法测定食品中苏丹红[J].广州化工,2011,39(19):75-79. |
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| 作者姓名： | 李春香 黄坚 马慧雪 张祥 |
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| 作者单位： | 中南大学化学化工学院,湖南长沙,410083 |
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| 基金项目： | 国家自然科学基金项目(No:2007AA022006)资助 |
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| 摘 要： | 建立了薄层层析-高效液相色谱联合技术测定食品中违禁添加物苏丹红Ⅰ、Ⅱ的新方法。采用硅胶板将苏丹红与杂质分离,在设定的色谱条件下测得浓度在0.1～20.0μg/mL范围内,峰面积（Y）与浓度（X）呈良好线性关系,苏丹红Ⅰ、的回归方程为Y1=-18785＋138962X,Y2=-2623＋131432X,相关系数R1=0.9997,R2=0.9998。将该法应用于辣椒粉、辣椒油、番茄酱等中的苏丹红Ⅰ、Ⅱ的检测。其检出限分别为0.024μg/g0、.027μg/g。
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| 关 键 词： | 薄层层析 高效液相色谱 硅胶板 苏丹红 |
Thin Layer Chromatography-High Performance Liquid Chromatographic Method for the Determination of Sudan Red in Food |
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| LI Chun-xiang,HUANG Jian,MA Hui-xue,ZHANG Xiang.Thin Layer Chromatography-High Performance Liquid Chromatographic Method for the Determination of Sudan Red in Food[J].GuangZhou Chemical Industry and Technology,2011,39(19):75-79. |
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| Authors: | LI Chun-xiang HUANG Jian MA Hui-xue ZHANG Xiang |
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| Affiliation: | LI Chun-xiang,HUANG Jian,MA Hui-xue,ZHANG Xiang(College of Chemistry and Chemical Engineering,Central South University,Hunan Changsha 410083,China) |
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| Abstract: | The new method of determining banned additives in the food such as Sudan red Ⅰ and Ⅱ was established with thin layer chromatography（TLC） and high performance liquid chromatography（HPLC） two methods combined.Sudan red and impurities can be separated by silica gel plate.Under the condition of chromatography in setting,the concentration in the 0.1~20.0 μg/mL range,the peak areas（Y） and concentration（X） present good linear relationship,Sudan red Ⅰ,Ⅱ regression equations Y1=-18785＋138962X,Y2=-2623＋131432X.Correlation coefficient R1=0.9997,R2=0.9998.Its application of determing the Sudan red Ⅰ and Ⅱ in chili powder,chili oil,and tomato sauce was studied,and got the detection limit of 0.024 μg/g,0.027 μg/g,respectively. |
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| Keywords: | thin layer chromatography high performance liquid chromatography self-made silica gel plate sudan red |
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