胃肠道癌肿患者外周血CD4^＋CD25^＋FOXP3^＋调节性T细胞的表达及临床意义

目的：研究胃肠道癌肿患者外周血CD4^＋CD25^＋FOXP3^＋调节性T（Treg）细胞的表达，并探讨其临床意义。方法：通过免疫荧光术及流式细胞仪检测20例胃癌患者及20例结肠癌患者外周血CD4^＋CD25^＋FOXP3^＋Treg细胞、CD4^＋CD25^＋high Treg细胞、CD4^＋T细胞及CD4^＋ CTLA-4^＋T细胞。结果：胃癌组、结肠癌组与健康献血者比较外周血CD4^＋CD25^＋FOXP3^＋ Treg细胞、CD4^＋CD25^＋high Treg细胞及CD4^＋CTLA-4^＋T细胞显著增多，CD4^＋T细胞显著减少；胃癌、结肠癌患者之间其外周血中CD4^＋CD25^＋FOXP3^＋Treg细胞、CD4^＋CD25^＋high Treg细胞、CD4^＋T细胞及CD4^＋CTLA-4^＋T细胞无显著差异。结论：胃肠道癌肿患者外周血CD4^＋CD25^＋FOXP3^＋Treg细胞显著高于健康献血者，这可能与胃肠道癌肿患者的免疫抑制和肿瘤的进展相关。

Peripheral regulatoryCD4^＋CD25^＋FOXP3^＋ T cells in patients with gastrointestinal malignancies

Objects ： To demonstrate the possible involvement of CD4^＋CD25^＋FOXP3^＋ regulatory T（ Tregl cells in immune system impairment in patients with gastrotestinal malignancies. METHODS： The CD4^＋CD25^＋FOXP3^＋ Treg cells, CD4^＋ CD25^＋high Treg cells,CD4^＋ T cells and CD4^＋ CTLA -4^＋ T cells were analyzed in peripheral blood in 20 patients with gastric cancer,20 patients with colon cancer by flow and immunofluorescence. RESULTS ： Compared with healthy controls, patients with gastric cancer and colon cancer had higher percentage of CD4^＋CD25^＋FOXP3^＋ Treg cells,CD4^＋ CD25^＋ high Treg cells and CD4^＋ CTLA -4 ^＋ T cells, had lower percent- age of CD4^＋ T cells. There were no significant differences between healthy volunteers and patients with gastric cancer or colon cancer. CONCLUSIONS ： The increased percent of CD4^＋CD25^＋FOXP3^＋ Treg cells may be related to immunosuppression and tumor progression in patients with gastrointestinal malignancies