| 纺锤体检查点蛋白Cenp-E在肝癌组织的表达及临床意义 |
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| 引用本文： | 刘斌,刘婧,刘卓然,汪翼,吴广.纺锤体检查点蛋白Cenp-E在肝癌组织的表达及临床意义[J].南华大学学报(医学版),2014,42(5):459-461. |
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| 作者姓名： | 刘斌 刘婧 刘卓然 汪翼 吴广 |
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| 作者单位： | 南华大学附属第二医院检验科，湖南 衡阳 421001 |
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| 基金项目： | 湖南省教育厅课题（No.13C796）. |
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| 摘 要： | 目的观察纺锤体检查点蛋白（Cenp-E）在肝癌组织和人类正常肝组织表达水平的差异，以探讨Cenp—E蛋白在肝癌细胞中的表达反临床意义。方法用荧光定量PCR和Westernblot分别检测Cenp．E基因和蛋白在肝癌组织和正常肝组织的表达水平。结果正常肝组织中Cenp-E基因mRNA水平（0．02378±0．00973）明显高于肝癌组织（0．01458±0．00873）；Cenp—E蛋白表达量在正常肝组织中（0．656±0．012）明显高于在肝癌组织中的表达（0．301±0．009），且二者有显著性差异。结论Cenp—E低表达在肝癌的发生过程中起重要作用。
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| 关 键 词： | 纺锤体检查点 Cenp—E基因 肝癌 |
| 收稿时间： | 2014/1/17 0:00:00 |
Expression of the Spindle Checkpoint Protein Cenp-E in the Human Hepatom Tissue and Its Clinical Significance |
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| LIU Bin,LIU Jing,LIU Zhuoran,et al.Expression of the Spindle Checkpoint Protein Cenp-E in the Human Hepatom Tissue and Its Clinical Significance[J].Journal of Nanhua University(Medical Edition),2014,42(5):459-461. |
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| Authors: | LIU Bin LIU Jing LIU Zhuoran |
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| Affiliation: | Clinical laboratory,the second affiliated hospital,university of south china,Hengyang,Hunan421001,China |
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| Abstract: | Objective To study the differential expression and its clinical significance of the spindle checkpoint protein Cenp-E gene between the human hepatoma and hepatic tissue. Methods Real-time fluorescent-quantitation PCR and Western blot were employed to detect the expressions of the Cenp-E mRNA and protein, respectively. Results The expression of the Cenp-E mRNA in human hepatic tissue（0. 023 78 ± 0.009 73 ） was higher than that in human hepatoma tissue（0.014 58 ±0.008 73）. The expression of the Cenp-E protein in human hepatic tissue（0. 656 ±0.012） was higher than that in human hepatoma tissue（0. 301 ± 0.009）. Conclusion Cenp-E gene low expression in human hepatocellular carcinoma plays an important role in the its developmental process. |
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| Keywords: | spindle checkpoint Cenp-E gene hepatoma |
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