Macroporous thin membranes for cell transplant in regenerative medicine |
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| Affiliation: | 1. Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain;2. Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valencia, Spain;1. National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China;2. Department of Biochemistry & Molecular Medicine, UC Davis Cancer Center, University of California Davis, Sacramento, CA 95817, USA;3. UNT System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA;1. German Center for Neurodegenerative Diseases (DZNE) Dresden, 01307 Dresden, Germany;2. Division of Neurodegenerative Diseases, Department of Neurology, Technische Universität Dresden, 01307 Dresden, Germany;3. Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, 01307 Dresden, Germany;4. Leibniz Institute of Polymer Research Dresden (IPF), Max Bergmann Center of Biomaterials Dresden (MBC), 01069 Dresden, Germany;1. Division of Chemical Engineering, College of Engineering, Hanyang University, Seoul 133-791, Republic of Korea;2. Amore-Pacific Co. R&D Center, 314-1 Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-729, Republic of Korea;3. Dept. of Bionano Technology, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Republic of Korea;4. Dept. of Applied Chemistry, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, Gyeonggi-do 426-791, Republic of Korea;1. Crystal Growth Centre, Anna University, Chennai 600 025, India;2. Variable Energy Cyclotron Centre (VECC), Kolkata 700064, West Bengal, India;3. Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 Valencia, Spain;4. Ciber en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 46022 Valencia, Spain;5. UGC-DAE Consortium for Scientific Research, Bidhan Nagar, Kolkata 700098, West Bengal, India |
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| Abstract: | The aim of this paper is to present a method to produce macroporous thin membranes made of poly (ethyl acrylate-co-hydroxyethyl acrylate) copolymer network with varying cross-linking density for cell transplantation and prosthesis fabrication. The manufacture process is based on template techniques and anisotropic pore collapse. Pore collapse was produced by swelling the membrane in acetone and subsequently drying and changing the solvent by water to produce 100 microns thick porous membranes. These very thin membranes are porous enough to hold cells to be transplanted to the organism or to be colonized by ingrowth from neighboring tissues in the organism, and they present sufficient tearing stress to be sutured with surgical thread. The obtained pore morphology was observed by Scanning Electron Microscope, and confocal laser microscopy. Mechanical properties were characterized by stress–strain experiments in tension and tearing strength measurements. Morphology and mechanical properties were related to the different initial thickness of the scaffold and the cross-linking density of the polymer network. Seeding efficiency and proliferation of mesenchymal stem cells inside the pore structure were determined at 2 h, 1, 7, 14 and 21 days from seeding. |
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| Keywords: | Macroporous thin membrane Poly (ethyl acrylate-co-hydroxyethyl acrylate) copolymer Mesenchymal stem cells Tearing stress |
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