| 拟南芥干旱诱导型启动子RD29A驱动花生AhNCED1基因双元表达载体的构建 |
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| 引用本文： | 万小荣,李玲. 拟南芥干旱诱导型启动子RD29A驱动花生AhNCED1基因双元表达载体的构建[J]. 植物生理学通讯, 2007, 43(2): 283-287 |
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| 作者姓名： | 万小荣 李玲 |
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| 作者单位： | 仲恺农业技术学院生命科学学院 广州510225(万小荣)，华南师范大学生命科学学院 广州510631(李玲) |
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| 基金项目： | 广东省自然科学基金;仲恺农业技术学院博士启动基金;广东省自然科学基金 |
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| 摘 要： | 从拟南芥基因组中克隆RD29A基因5'-侧翼520bp启动子区域序列,生物信息学分析表明,该启动子片段中存在脱水胁迫响应元件(DRE)、ABA响应元件(ABRE)、TATA-box、CAAT-box等顺式作用元件。构建了干旱诱导型启动子AtRD29Ap驱动花生AhNCED1基因的植物双元表达载体pAtRD29Ap::AhNCED1。
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| 关 键 词： | RD29A基因启动子 NCED基因 双元载体构建 |
| 收稿时间： | 2007-01-08 |
| 修稿时间： | 2007-03-16 |
Construction of Binary Vector Harboring Peanut AhNCED1 Gene Driven by Drought-inducible Promoter of RD29A Gene from Arabidopsis |
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| WAN Xiao-Rong,LI Ling. Construction of Binary Vector Harboring Peanut AhNCED1 Gene Driven by Drought-inducible Promoter of RD29A Gene from Arabidopsis[J]. Plant Physiology Communications, 2007, 43(2): 283-287 |
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| Authors: | WAN Xiao-Rong LI Ling |
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| Abstract: | In this paper we report the cloning of 5'-flanking promoter sequence of drought-inducible RD29A gene from Arabidopsis and its fusion with peanut AhNCED1 gene. The 520-bp promoter sequence was ana- lyzed bioinformatically in the database of plant cis-acting regulatory element (PlantCARE). The result showed that there were several important cis-acting elements, including TATA-box, CAAT-box, DRE (response to dehydration), ABRE (response to ABA), in the 520-bp promoter region. The binary vector harboring AhNCED1 gene driven by AtRD29A promoter was further constructed to be used in coming plant transgene. |
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| Keywords: | RD29A gene promoter nine-cis-epoxycarotenoid dioxygenase (NCED) gene binary vector con- struction |
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