断藤益母汤对佐剂性关节炎大鼠骨破坏的干预作用

目的:探讨断藤益母汤抗炎、抗骨质破坏的作用,及其抑制骨质破坏的机制。方法:72只SD大鼠分成9组,即正常组、模型组、甲氨喋呤组(MTX,1.06 mg·kg-1)、南蛇藤高、中、低剂量组(47,23.5,4.7 g·kg-1)、断藤益母汤高、中、低剂量组(94,47,9.4 g·kg-1)。除正常组外,其余各组给药完全弗氏佐剂建立模型,造成佐剂性关节炎(AA)模型后,MTX每周ig给药1次,其余各组每天ig给药1次,连续36 d,观察足肿胀度,检测血清I型胶原羧基末端肽(CTX-I),I型前胶原氨基端前肽(PINP)水平,组织病理切片及踝关节X射线。结果:与正常组比较,模型组足肿胀度、血清CTX-I水平和踝关节X射线评分均明显增高(P0.01),血清PINP水平无统计学差异;与模型组比较,各给药组足肿胀度、血清CTX-I水平和踝关节X射线评分均明显降低(P0.05,P0.01),且断藤益母汤各剂量组均优于单方南蛇藤各剂量组及氨甲喋呤组,血清PINP水平无统计学差异。病理结果显示,各给药组大鼠关节滑膜细胞增生、炎症细胞浸润、血管翳形成、软骨破坏均较模型组减轻,且断藤益母汤组对滑膜增生和软骨破坏的改善较南蛇藤组效果更优。结论:断藤益母汤能减轻AA大鼠关节炎、抑制骨破坏的作用机制与抑制血清CTX-I水平有关。

Effect of Duanteng Yimu Decoction on Bone Destruction in Rats with Adjuvant Arthritis

Objective: To explore the effect of duanteng Yimu decoction (DTYM) on anti-inflammation and counteracting bone destruction, and to investigate its mechanism of counteracting bone destruction of adjuvant arthritis in rats. Method: Seventy-two SD rats were divided into nine groups;the normal group, the model group, the methotrexate group (MTX, 1.06 mg · kg^-1), the high-, medium-, low-dose celastrus orbiculatus groups (47, 23.5, 4.7 g · kg^-1) and the high-, medium-, low-dose DTYM groups (94, 47, 9.4 g · kg^-1). The rats except the normal group were induced by injecting complete Freund's adjuvant to establish adjuvant arthritis rat model. After intragastrical administration of the corresponding medicines once daily for 36 days, the feet swelling degrees, ankle X-ray, C-terminal telopeptide of type I collagen (CTX-I) and N-terminal propeptide of type I collagen (PINP) serum concentration, and ankle pathological were observed. Result: Compared with the normal group, the feet swelling degrees, CTX-I and ankle X-ray score increased significantly in the model group (P<0.01). Compared with the model group, the feet swelling degrees, CTX-I and ankle X-ray score decreased significantly in all treatment groups (P<0.01). No statistically significant differences in PINP were found between any two groups. Pathological study showed that there were less synovial cell hyperplasia, inflammatory cell infiltrations, pannus formation and cartilage destruction in all treatment groups. Moreover, the above results were better in the DTYM groups. Conclusion: The mechanism of DTYM decoction in improving arthritis and bone destruction may be related to inhibiting the level of CTX-I.