紫茄皮红色素抗氧化活性的研究

为了研究紫茄皮红色素的抗氧化活性，为其应用奠定理论基础。以紫茄皮为原料，以抗坏血酸、柠檬酸为对照，以Fenton反应产生羟基自由基(?OH)，邻苯三酚自氧化反应产生超氧阴离子自由基(O2-?)为试验模型，采用紫外分光光度计测定紫茄子皮红色素对羟基自由基、超氧阴离子自由基的清除作用；以猪油作底物，采用烘箱贮藏法测定油脂的POV值，研究了紫茄皮红色素对油脂的抗氧化活性。结果表明：紫茄子皮红色素对上述自由基均有清除作用，对羟基自由基清除效果比柠檬酸、抗坏血酸好，且清除效果随着浓度的增大而增强，当浓度大于0.5 g/100 mL，对羟基自由基清除能力趋于稳定；对超氧阴离子自由基清除效果比柠檬酸、抗坏血酸好，最大抑制率为91%，当浓度大于0.6 g/100 mL后，对邻苯三酚自氧化的抑制作用趋于稳定；抑制脂质过氧化的程度强于柠檬酸和抗坏血酸，在0.7 g/mL的浓度下，协同增效作用趋于稳定。紫茄皮红色素在一定浓度范围内有很强的抗氧化活性。

Research on the Antioxidant Activity of Red Pigment from Purple Eggplant Peel

The aims were to study on the antioxidant activity oI red pigment Irom purple eggplant peel anti lay the theoretical foundation for its application. In this paper, the purple eggplant skin was used as raw materials and ascorbie acid, citric acid were used as contrasts, hydroxyl radical （- OH） from Fenton reaction and superoxide anion （O2-·） from pyrogallol autoxidation reaction were used as the test model and the radical scavenging effect of purple eggplant skin red pigment against superoxide anion and hydroxyl radical were measured by using UV spectrophotometer. The antioxidant activity of red pigment eggplant purple skin against fat oxidation was studied when lard was used as substrate and PO V value of oil was determined by the oven storage method. Red pigment from purple eggplant skin could effect on free radicals above and its effect on hydroxyl radical scavenging was better than citric acid, ascorbic acid and scavenging effect was increased with the concentration increasing and the hydroxyl radical scavenging capacity tended to the stable situation when the concentration was greater than 0.5 g/100 mL; its effect was better than citric acid, aseorbic acid, with the maximum inhibition rate 91% and the pyrogallol autoxidation effected to stable inhibition when the concentration was above 0.6 g/100 mL; the degree of inhibition of lipid peroxidation was stronger than citric acid and ascorbic acid and synergies tended to stabilize when the concentration was 0.7 g/mL. Purple eggplant skin red pigment in a certain concentration range had strong antioxidative activity.