| 一株鸭坦布苏病毒的分离鉴定及全基因组序列分析 |
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| 引用本文： | 刘胜旺,张玥,刘晓丽,李云霞,韩宗玺,邵昱昊,孔宪刚.一株鸭坦布苏病毒的分离鉴定及全基因组序列分析[J].东北农业大学学报,2013(3):72-78. |
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| 作者姓名： | 刘胜旺 张玥 刘晓丽 李云霞 韩宗玺 邵昱昊 孔宪刚 |
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| 作者单位： | 东北农业大学生命科学学院;中国农业科学院哈尔滨兽医研究所禽病研究室 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目(201203056) |
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| 摘 要： | 2010年我国爆发了使蛋鸭产蛋量急剧下降、由一种鸭黄病毒引起的疾病,通过对来自山东某发病鸭场的病料进行分离,得到实验室分离株Du/CH/LSD/110128。对其进行全基因组序列测定分析,确定该株病毒全基因组含有一个开放阅读框,编码一个由3 426个氨基酸组成的多聚蛋白。将试验测得株Du/CH/LSD/110128株与黄热病毒(Yellow fever virus)、登革热病毒(Dengue virus)、伊利乌斯脑炎病毒(Ilheus virus)、西尼罗病毒(West Nilevirus)、巴格扎病毒(Bagaza virus)和其他一些已公布序列的坦布苏病毒进行核酸同源性比较和遗传进化树分析,发现Du/CH/LSD/110128株与巴格扎病毒亲缘关系较近但介于病毒种的水平上,与其他株坦布苏病毒核酸同源性在87%以上,可以确定Du/CH/LSD/110128株属于新型鸭坦布苏病毒(Duck Tembusu virus)。
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| 关 键 词： | 鸭坦布苏病毒 分离鉴定 基因组 分离鉴定 |
Isolation and genomic sequence analysis of Duck Tembusu virus |
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| LIU Shengwang,ZHANG Yue,LIU Xiaoli,LI Yunxia,HAN Zongxi,SHAO Yuhao,KONG Xiangang.Isolation and genomic sequence analysis of Duck Tembusu virus[J].Journal of Northeast Agricultural University,2013(3):72-78. |
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| Authors: | LIU Shengwang ZHANG Yue LIU Xiaoli LI Yunxia HAN Zongxi SHAO Yuhao KONG Xiangang |
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| Affiliation: | 1.School of Life Sciences,Northeast Agricultural University,Harbin 150030,China;2.Avian Diseases,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | During investigations into the outbreak of duck viral infection in 2010 in China,with a severe drop in egg production,a flavivirus was isolated from the affected ducks from Shandong Province.It was characterized as a Duck Tembusu virus(DTMUV).In this study,we obtained a complete genome sequence of DTMUV named Du/CH/LSD/110128.Du/CH/LSD/110128 strain contains a single open reading frame(ORF) encoding a polyprotein is comprised of 3 426 amino acids.The different genomic regions of Du/CH/LSD/110128 strain were also compared with those of flaviviruses including Yellow fever virus,Dengue virus,Ilheus virus,West Nile virus,Bagaza virus and other Tembusu viruses.Du/CH/LSD/110128 strain demonstrated the higher similarity to Bagaza virus.The result of entire ORF scanning shows that Du/CH/LSD/110128 was characterized as a new duck Tembusu virus. |
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| Keywords: | DTMUV isolation genome isolation and dentifacation |
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