Protective efficacy of an inactivated Eurasian avian-like H1N1 swine influenza vaccine against homologous H1N1 and heterologous H1N1 and H1N2 viruses in mice |
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| Affiliation: | 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, PR China;2. National Avian Influenza Reference Laboratory, Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Harbin 150001, PR China;1. College of Wildlife Resources, Northeast Forestry University, Harbin 150040, People’s Republic of China;2. Harbin Veterinary Research Institute, Harbin 150001, People’s Republic of China;3. College of Animal Science and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, People’s Republic of China;1. Tianjin Animal Husbandry and Veterinary Research Institute, Tianjin 300381,China;2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;1. Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China;2. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun 130122, China;3. Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250132, China;4. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;5. Department of Microbiology, University of Wisconsin-La Crosse, La Crosse, Wisconsin, 54601, USA;6. Basic Medical College,Changchun University of Traditional Chinese Medicine, Changchun, 130117, China;7. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China;8. Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China;1. Department of Biology, Georgia Southern University, Statesboro, GA, USA;2. Virus and Prion Research Unit, National Animal Disease Center, USDA-ARS, Ames, IA, USA;3. Fogarty International Center, National Institutes of Health, Bethesda, MD, USA;4. School of Human Evolution and Social Change, Arizona State University, Tempe, AZ, USA;5. Department of Zoology, University of Cambridge, Cambridge, United Kingdom;6. National Veterinary Services Laboratories, USDA-APHIS-VS-STAS, Ames, IA, USA |
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| Abstract: | Eurasian avian-like H1N1 (EA H1N1) swine influenza viruses are prevalent in pigs in Europe and Asia, but occasionally cause human infection, which raises concern about their pandemic potential. Here, we produced a whole-virus inactivated vaccine with an EA H1N1 strain (A/swine/Guangxi/18/2011, SW/GX/18/11) and evaluated its efficacy against homologous H1N1 and heterologous H1N1 and H1N2 influenza viruses in mice. A strong humoral immune response, which we measured by hemagglutination inhibition (HI) and virus neutralization (VN), was induced in the vaccine-inoculated mice upon challenge. The inactivated SW/GX/18/11 vaccine provided complete protection against challenge with homologous SW/GX/18/11 virus in mice and provided effective protection against challenge with heterologous H1N1 and H1N2 viruses with distinctive genomic combinations. Our findings suggest that this EA H1N1 vaccine can provide protection against both homologous H1N1 and heterologous H1N1 or H1N2 virus infection. As such, it is an excellent vaccine candidate to prevent H1N1 swine influenza. |
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| Keywords: | Swine influenza virus Avian-like H1N1 Vaccine |
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