香菇多糖的免疫调节作用

研究香菇多糖(LTN)的免疫调节作用。结果表明,LTN1及5mg·kg^-1·d^-1×6,ip可促进正常小鼠由ConA(2.5mg·kg^-1)刺激的脾脏T淋巴细胞增殖反应。1,5及10mg·kg^-1·d^-1×8或5,ip能分别纠正由环磷酰胺(Cy,200mg·kg^-1和80mg·kg^-1,ip)诱导的免疫亢进或低下状态。此外,LTN(1,5和10mg·kg^-1·d^-1×6,ip),促使小鼠胸腺L3T4+(Th)和Lyt2+(Ts)细胞数减少,外周脾脏L3T4+和Lyt2+细胞数增加,腹腔巨噬细胞释出肿瘤坏死因子(TNF)也明显增加。这些作用均以LTN5mg·kg^-1·d^-1作用最佳。提示LTN可能通过影响T细胞及其亚型,促进TNF活性调节机体的免疫功能。

THE IMMUNOMODULATORY EFFECT OF LENTINAN

Lentinan(LTN) was extracted from Lentinus edodes (Berk)Sing with molecularweight of 5×10⁵.The effects of lentinan on cellular immune function were studied in vivo bymeasuring the cellular delayed type hypersensitivity(DTH)to dinitrofluorobenzene (DNFB)incyclophosphamide(Cy)-comprised mice.The effect of lentinan on T lymphocyte proliferation toCon A on splenocytes and T lymphocyte subpopulations on thymocytes and on splenocytes from normalmice were also evaluated.Moreover, the effect of LTN on production of tumor necrosis factor(TNF) from murine peritoneal macrophage was also tested.LTN was administrated at doses of 1,5and 10 mg·kg^-1·d^-1.The following results were observed :LTN administration(×6) augmentedthe T lymphocyts proliferation to Con A in normal mice; LTN restorated the DTH to DNFB impairedby single Cy(200 mg· kg^-1 and 80 mg· kg^-1 ,ip)after using LTN for 8 or 5d;LTN administration(×6)either decreased the nercentage of L3T4⁺(Th),Lyt2⁺(Ts)in thymocytes or increased thepercentage of L3T4⁺,Lyt2⁺ in splenocytes;LTN(×6)administration elicited release of TNF fromMth in the presence of lipoplysaccharide(LPS).These results indicate that the immunomodulatingeffect of LTN mav be relevant to change of T cell subpopulation and increase of TNF production.