雷公藤多苷对青春期大鼠睾丸组织及c-kit表达的影响

目的：探讨雷公藤多苷对青春期大鼠睾丸组织的近、远期影响及机制。方法：5周龄雄性Sprague-Dawley（SD）大鼠48只，随机分为对照组、小剂量和大剂量雷公藤多苷组（每组16只），分别给予1%羧甲基纤维素钠、雷公藤多苷小剂量（每日6 mg/kg）和大剂量（每日12 mg/kg）灌胃4周，观察停药24 h和4周后大鼠的睾丸组织学改变及c-kit表达。结果：停药24 h，两个雷公藤多苷组睾丸生精细胞均减少、c-kit表达下调（P0.05），大剂量组仍表现为生精上皮萎缩、间质水肿，睾丸重量、生精细胞 c-kit 表达下降（P<0.01）。结论：雷公藤多苷可致青春期大鼠睾丸组织损伤，小剂量引起睾丸组织近期可逆性损伤；大剂量可致睾丸组织远期损伤，提示睾丸间质病变可能是雷公藤多苷致青春期大鼠睾丸组织远期损伤的原因之一。

Effects of multi-glycosides of tripterygium wilfordii on histological structures and c-kit expression in testes of pubertal rats

Objective To study the short-and long-term effects of multi-glycosides of tripterygium wilfordii(GTW)on the histological structures of testes in pubertal rats and possible mechanisms.Methods Forty-eight 5-week-old male Sprague-Dawley rats were randomly intragastrically administered with low-does GTW(6 g/kg daily)and high-does GTW(12 mg/kg daily) or 1% sodium carboxymethylcellulose(6 mL/kg,control group) for four weeks.The testes were sampled for detecting histological structures and c-kit expression by immunohistochemistry 24 hrs and four weeks after drug discontinuance.Results The number of spermatogenic cells and the expression of c-kit in testes were reduced in the two GTW treatment groups 24 hrs and 4 weeks after drug discontinuance compared with those in the control group(P<0.05).Four weeks after drug discontinuance atrophy and interstitial edema of seminiferous epitheliumin in testes were observed,and the testis weight and the expression of c-kit in testes were reduced in the high-does GTW group compared with those in the control group(P<0.01).There were no significant differences in the parameters observed between the low-dose GTW and the control group 4 weeks after drug discontinuance.Conclusions GTW has adverse effects on testes in a dose-dependent manner in puberty rats.Low-dose GTW may cause reversible short-term injuries to testis tissues.The damage of the interstitial tissue of testes induced by high-dose GTW may be one of the causes of long-term injuries of testes.