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Proteolysis in ultra-filtered and conventional Iranian white cheese during ripening
Authors:JAVAD HESARI  MOHAMED R EHSANI  MOHAMED A E MOSAVI  PAUL L H McSWEENEY
Affiliation:Department of Food Science and Technology, Tabriz University, PO Box 51666, Tabriz, Iran,;Department of Food and Nutritional Sciences, University College, Cork, Ireland,;Department of Food Science and Technology, Tehran University, Tehran, Iran
Abstract:Conventional and ultra-filtered (UF) Iranian white cheeses were made with almost identical gross chemical composition and the extent and characteristics of proteolysis were studied during ripening. UF cheeses exhibited a lower rate of development of pH 4.6-soluble nitrogen than conventional cheeses. The rates of degradation of αs1-casein and particularly β-casein were lower in UF cheeses than in conventional cheeses. Plasmin activity was lower in UF cheeses than that in conventional cheese, whereas coagulant activity was higher in the former. Noticeable qualitative and quantitative differences were observed in reverse-phase high performance liquid chromatography (RP-HPLC) peptide profiles between UF and conventional white cheeses and chemometric analysis of peak height data distributed the cheeses into two separate groups. The levels of free amino acids in UF cheeses were lower than in conventional cheeses. Lower peptide degradation and production of amino acids suggested slower ripening, which may have been associated with the weak aroma development characteristic of UF cheeses.
Keywords:Cheese ripening  Iranian white cheese  Proteolysis  UF
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