结肠癌和癌前病变演化过程中细胞凋亡与增殖的关系

目的 ]阐明结肠癌和癌前病变中细胞凋亡与增殖的关系 .方法 ]应用免疫组织化学亲和素 过氧化物酶法检测 148例结肠病变标本中增殖细胞核抗原蛋白的表达 ;并用末端脱氧核苷酸转移酶介导的缺口末端标记技术检测 75例标本中的凋亡细胞 .结果 ]增殖细胞核抗原蛋白在结肠癌和结肠腺瘤中的表达率较正常组织高 ,以结肠腺瘤为分界点 ,前半阶段凋亡指数逐渐上升 ,后半阶段则下降 ,而细胞增殖指数在整个演进过程中呈上升趋势 .结论 ]增殖细胞核抗原在结肠癌和癌前病变中的表达率增高 ,与分化、浸润深度和有无淋巴结转移无关 ,但增殖细胞核抗原过表达与结肠癌分化有关 ,伴有丝分裂指数的升高 .

Analysis of the relationship between apoptosis and proliferation in carcinoma and precancerous lesion of colon

OBJECTIVE To investigate the relationship between apoptosis and proliferation in carcinoma and precancerous lesion of colon. METHODS The expression of proliferating cell nuclear antigen was detected by immunohistochemical method in 148 cases of pathologic changes of colon and cell apoptosises were detected by TUNEL in 75 cases of samples. RESULTS The expression of proliferating cell nuclear antigen was related with both apoptosis and proliferating index, positive expressive ratio of proliferating cell nuclear antigen was higher in carcinoma and adenoma of colon than in normal tissues, apoptosis index increased in anterior partial phase and descended in posterior partial phase,and proliferating index and increased in whole procession. CONCLUSION The positive expressive ratio of proliferating cell nuclear antigen was high in carcinoma and adenoma of colon and was no related with differentiation, infiltrated depth and metastasis of lymph node, but the over expression of proliferating cell nuclear antigen was related with differentiation of carcinoma of colon.