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| EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析 | |
| 引用本文： | 陈洋,高兰英,邵艳军,张增艳.EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析[J].核农学报,2011,25(4):617-621,651. |
| 作者姓名： | 陈洋 高兰英 邵艳军 张增艳 |
| 作者单位： | 1. 河北农业大学生命科学学院,河北保定071001／中国农业科学院作物科学研究所／农作物基因资源与基因改良国家重大科学工程,北京100081 2. 中国农业科学院作物科学研究所／农作物基因资源与基因改良国家重大科学工程,北京,100081 3. 河北农业大学生命科学学院,河北保定,071001 |
| 基金项目： | 国家“863”计划课题(2006AA10A104) |
| 摘 要： | 抗黄矮病的小麦-中间偃麦草易位系YW642携带中间偃麦草抗黄矮病基因Bdv2。用化学诱变剂甲基磺酸乙酯（EMS）处理抗黄矮病的小麦-中间偃麦草易位系YW642的种子6000粒,从M2中筛选出32类不同性状的突变体73株,表型变异率约为7.38%。对感黄矮病突变体的M3、M4代继续进行鉴定,证明18个感黄矮病突变体的突变... |
| 关 键 词： | 小麦 甲基磺酸乙酯(EMS) 突变体 小麦黄矮病抗性 |
| 收稿时间： | 2011-02-25 |
PHENOTYPIC IDENTIFICATION AND MOLECULAR ANALYSIS OF A WHEAT LINE MUTANT YW642 INDUCED BY EMS |
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| CHEN Yang, GAO Lan-yin SHAO Yan-jun ZHANG Zeng-yan.PHENOTYPIC IDENTIFICATION AND MOLECULAR ANALYSIS OF A WHEAT LINE MUTANT YW642 INDUCED BY EMS[J].Acta Agriculturae Nucleatae Sinica,2011,25(4):617-621,651. | |
| Authors: | CHEN Yang GAO Lan-yin SHAO Yan-jun ZHANG Zeng-yan |
| Affiliation: | CHEN Yang1,2 GAO Lan-yin2 SHAO Yan-jun1 ZHANG Zeng-yan2(1.College of Life Science,Hebei Agricultural University,Baoding,Hebei 071001,2.National Key Science Facility of Crop Gene Resources and Gene Improvement/Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081) |
| Abstract: | The wheat-Thinopyrum intermedium translocation line YW642 carries a T.intermedium-derived gene resistant to barley yellow dwarf virus(BYDV),Bdv2.The wheat line YW642 was treated by EMS(ethyl methane sulfonate) for the construction of the mutant library.After the selection from M2 generation,73 mutant lines with 39 types of traits were obtained with the mutation rate of 7.38%.Among them,18 mutants with BYDV sensitivity were identified through verifications of M3 to M4 generations,but their resistances were r... |
| Keywords: | wheat ethyl methane sulfonate(EMS) mutant resistance to barley yellow dwarf virus |
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