葡糖醋杆菌FBFS97产苯乳酸的液态发酵条件优化

该试验以葡糖醋杆菌（Gluconacetobacter sp.）FBFS97为出发菌株，在单因素试验的基础上，通过Plackett-Burman试验、最陡爬坡试验、Box-Behnken响应面法优化菌株产苯乳酸的液态发酵条件。结果表明，优化后的发酵条件为果糖100 g/L、酵母粉40 g/L、K2HPO4 1.2 g/L，pH 4.4，接种量5%（V/V），装液量24 mL/250 mL，于28 ℃、150 r/min培养8 d。在此优化条件下，苯乳酸产量为217 mg/L，是优化前的3.52倍。

Optimization of liquid-state fermentation conditions for phenyllactic acid production by Gluconacetobacter sp. FBFS97

Using Gluconacetobacter sp. FBFS97 as starting strain, on the basis of single factor test, the liquid-state fermentation conditions for phenyllactic acid production by Gluconacetobacter sp. FBFS97 were optimized by Plackett-Burman experiment, steepest climbing experiment and Box Benhnken response surface method. The results showed that the optimal fermentation conditions were fructose 100 g/L, yeast powder 40 g/L, K2HPO4 1.2 g/L, pH 4.4, inoculum 5% (V/V), loaded liquid 24 ml/250 ml, culture temperature 28 ℃, rotating speed 150 r/min, and fermentation time 8 d. Under the optimal fermentation conditions, the phenyllactic acid production was 217 mg/L, which was 3.52 times that of before optimization.