酰肼介导的抗体定向偶联策略提高免疫层析试纸条检测性能

以酰肼功能团修饰的胶体金作为试纸条的标记材料，利用酰肼基团可特异性地与抗体Fc片段醛基发生亲核加成反应的特性，实现抗体在胶体金纳米粒子（gold nanoparticles，AuNPs）表面的定向偶联。对比于碳二亚胺介导的共价偶联法，酰肼功能团介导的定向偶联需要更少的抗体标记量，更短的偶联时间，酰肼化胶体金免疫层析试纸条（hydrazided gold nanoparticles-based immunochromatographic assay，hAuNPs-ICA）检测玉米赤霉烯酮（zearalenone，ZEN）展示出更好的检测灵敏度；将hAuNPs-ICA检测ZEN加标的玉米样品，结果显示加标回收率介于83.1%～118.0%，批内、批间变异系数为3.9%～13.1%，表明hAuNPs-ICA具有较好的精密度和准确性。

Hydrazide-Assisted Directional Antibody Conjugation of Gold Nanoparticles to Enhance the Performance of Immunochromatographic Assay

Anti-zearalenone monoclonal antibodies (mAbs) were directionally conjugated on the surface of hydrazide group-functionalized gold nanoparticles because of the nucleophilic addition reaction between the aldehyde groups of the antibody Fc region and the hydrazide groups on gold nanoparticles (AuNPs). Compared with the carbodiimide-mediated covalent coupling method, the proposed directional coupling strategy showed several advantages such as use of less amounts of labeled antibody and shorter reaction time. The hydrazided gold nanoparticles-based immunochromatographic assay (hAuNPs-ICA) showed higher sensitivity for ZEN detection. Additionally, when hAuNPs-ICA was used to analyze ZEN-spiked maize samples, the average recoveries ranged from 83.1% to 118.0% with intra- and inter-batch coefficients of variation ranging from 3.9% to 13.1%, indicating good accuracy and precision.