醛、醇脱氢酶基因多态性与三氯乙烯药疹样皮炎易感性的关系

目的研究醛脱氢酶、醇脱氢酶基因多态性与三氯乙烯药疹样皮炎易感性的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,比较108例三氯乙烯药疹样皮炎病人和145例健康三氯乙烯接触工人醛脱氢酶2(ALDH2)、醇脱氢酶2(ADH2)和醇脱氢酶3(ADH3)的基因多态性分布,并计算相对危险度(OR)。结果ADH2和ADH3基因型分布在病人与接触对照工人中无显著性差异;ALDH2变异型基因(ALDH2*1/*2+ALDH2*2/*2)频率在病人中显著低于接触对照工人(分别为27·8%和43·4%,P=0·011),使三氯乙烯药疹样皮炎的危险性显著降低(OR=0·50,95%CI=0·29~0·85)。结论高活性ALDH2可能是导致三氯乙烯药疹样皮炎个体易感性差异的原因之一。

Polymorphisms of aldehyde and alcohol dehydrogenase genes associated with susceptibility to trichloroethylene-induced medicamentosa-like dermatitis

Objective investigate the genetic polymorphisms of aldehyde dehydrogenase(ALDH) and alcohol dehydrogenase(ADH),major enzymes involving the trichloroethylene(TCE) metabolism,associated with susceptibility to TCE-induced medicamentosa-like dermatitis. Methods The study included 108 patients with TCE-induced medicamentosa-like dermatitis and 145 healthy controls exposed to TCE who were engaged in the same workplace,and frequency matched by sex and age. The genotypes of ADH2,ADH3 and ALDH2 were analyzed by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP),and distribution of genotype and odds ratio were calculated. Results There were no differences in the frequencies of genotypes of ADH2 and ADH3 between cases and exposed controls. The frequency of heterozygous ALDH2*1/*2 plus homozygous ALDH2*2/*2 in cases was significantly lower than that in exposed controls(27.8% vs 43.4%,P=0.011),and it decreased the risk of TCE-induced medicamentosa-like dermatitis (OR=0.50,95% CI = 0.29-0.85). Conclusion The active ALDH2 might be one of the factors influencing the individual susceptibility to TCE- induced medicamentosa-like dermatitis.