| 水稻转座子Pong特异性RNAi载体TPAS的构建和农杆菌介导的遗传转化条件的优化 |
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| 引用本文： | 高立宏,张春玉,柴娜,路云侠,刘宝,刘立侠.水稻转座子Pong特异性RNAi载体TPAS的构建和农杆菌介导的遗传转化条件的优化[J].东北师大学报(自然科学版),2011(4):122-127. |
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| 作者姓名： | 高立宏 张春玉 柴娜 路云侠 刘宝 刘立侠 |
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| 作者单位： | 长春师范学院生命科学学院;东北师范大学生命科学学院;长春职业技术学院; |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(2005CB120805) |
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| 摘 要： | 为研究水稻转座子Pong的功能,构建了Pong的特异RNAi表达载体TPAS,并利用农杆菌介导法将这个载体转化到水稻的成熟胚愈伤组织,获得了PCR阳性的再生植株.同时通过对农杆菌侵染途径、侵染浓度和侵染时间等的研究,建立并优化了水稻的遗传转化程序,结果表明:在农杆菌侵染成熟胚愈伤组织的时间为3 min,共培养2～4 d...
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| 关 键 词： | 水稻 转座子 RNAi 农杆菌介导 遗传转化 |
Specific RNAi vector construction of transposon Pong in rice and Agrobacterium tumefaciens-mediated genetic transformation optimization |
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| GAO Li-hong,ZHANG Chun-yu,CHAI Na,LU Yun-xia,LIU Bao,LIU Li-xia.Specific RNAi vector construction of transposon Pong in rice and Agrobacterium tumefaciens-mediated genetic transformation optimization[J].Journal of Northeast Normal University (Natural Science Edition),2011(4):122-127. |
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| Authors: | GAO Li-hong ZHANG Chun-yu CHAI Na LU Yun-xia LIU Bao LIU Li-xia |
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| Affiliation: | GAO Li-hong1,2,ZHANG Chun-yu1,3,CHAI Na1,LU Yun-xia1,LIU Bao1,LIU Li-xia1(1.School of Life Sciences,Changchun Teachers College,Changchun 130032,China,2.School of Life Sciences,Northeast Normal University,Changchun 130024,3.Changchun Vocational Institute of Technology,Changchun 130033,China) |
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| Abstract: | To study the function of rice transposon Pong,the research constructed the specific RNAi expressed vector TPAS of transposon Pong in rice,and transferred the vectors into the mature embryo callus of the rice cultivar Matsumae,and then obtained the regeneration of the positive PCR results by Agrobacterium tumefaciens-mediated method.To establish and optimize genetic transformation process,the different infected ways,concentrations and time of Agrobacterium were chosen.The result showed that:the infection tim... |
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| Keywords: | rice transposon RNAi tumefaciens-mediated genetic transformation |
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