长链非编码RNA NR2F2-AS1对胃癌细胞增殖、凋亡的影响

目的:研究lncRNA NR2F2-AS1对胃癌细胞增殖、凋亡的影响，并探讨其机制。方法:运用qRT-PCR法检测细胞中 NR2F2-AS1、miR-425-5p的mRNA表达情况；将pcDNA组（转染pcDNA）、pcDNA-NR2F2-AS1组（转染pcDNA-NR2F2-AS1）、sh-NR2F2-AS1组(转染sh-NR2F2-AS1)、sh-NC组(转染sh-NC)、anti-miR-NC组（转染anti-miR-NC）、anti-miR-425-5p组（转染anti-miR-425-5p）、pcDNA-NR2F2-AS1+miR-NC组（共转染pcDNA-NR2F2-AS1和miR-NC）、pcDNA-NR2F2-AS1+miR-425-5p组（共转染pcDNA-NR2F2-AS1和miR-425-5p mimics）转染至MGC-803、MKN-45细胞；MTT法检测细胞的增殖；流式细胞术检测细胞的凋亡；双荧光素酶报告基因检测实验检测细胞的荧光活性。结果:与正常胃黏膜上皮细胞GES-1相比，胃癌细胞MGC-803、MKN-45中NR2F2-AS1的表达显著降低，miR-425-5p的表达显著升高；过表达NR2F2-AS1、抑制miR-425-5p均可抑制胃癌细胞增殖，促进细胞凋亡；miR-425-5p可抑制野生型NR2F2-AS1的MGC-803、MKN-45细胞的荧光活性；过表达miR-425-5p可逆转过表达NR2F2-AS1对MGC-803、MKN-45细胞增殖和凋亡的作用。结论:lncRNA NR2F2-AS1可抑制胃癌细胞的增殖，促进细胞凋亡，其机制可能与靶向miR-425-5p有关，将可为胃癌的治疗提供新靶点。

Effect of long non-coding RNA NR2F2-AS1 on proliferation and apoptosis of gastric cancer cells

Objective:To study the effects of lncRNA NR2 F2-AS1 on proliferation and apoptosis of gastric cancer cells and to explore its mechanism.Methods:qRT-PCR was used to detect the mRNA expression of NR2 F2-AS1 and miR-425-5 p in the cells.pcDNA group(transfected pcDNA),pcDNA-NR2 F2-AS1 group(transfected pcDNA-NR2 F2-AS1),sh-NR2 F2-AS1 group(transfected sh-NR2 F2-AS1),sh-NC group(transfected sh-NC),anti-miR-NC group(transfected anti-miR-NC),anti-miR-425-5 p group(transfected anti-miR-425-5 p),pcDNA-NR2 F2-AS1+miR-NC group(co-transfected pcDNA-NR2 F2-AS1 and miR-NC),pcDNA-NR2 F2-AS1+miR-425-5 p(co-transfected pcDNA-NR2 F2-AS1 and miR-425-5 p mimics) were transfected into MGC-803,MKN-45 cells.Proliferation was detected by MTT assay.Flow cytometry detected cell apoptosis.Dual luciferase reporter assay detected fluorescence activity of cells.Results:Compared with GES-1,a normal gastric mucosal epithelial cell,the expression of NR2 F2-AS1 was significantly decreased in gastric cancer cells MGC-803,MKN-45,and the expression of miR-425-5 p was significantly increased. Overexpression of NR2 F2-AS1 and inhibition of miR-425-5 p can inhibit the proliferation of gastric cancer cells and promote apoptosis.miR-425-5 p can inhibit the fluorescence activity of wild-type NR2 F2-AS1 MGC-803,MKN-45 cells. Overexpression of miR-425-5 p can reverse the overexpression of NR2 F2-AS1 to MGC-803,MKN-45 cell proliferation and apoptosis.Conclusion:Long-chain non-coding RNA NR2 F2-AS1 can inhibit the proliferation of gastric cancer cells and promote apoptosis.The mechanism may be related to targeting miR-425-5 p,which will provide a new target for the treatment of gastric cancer.