针刺对多发梗塞性痴呆大鼠海马cAMP和cGMP含量的影响

目的:观察针刺足三里穴对多发梗塞性痴呆大鼠第二信使环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)含量的影响。方法:采用栓子注入法制备大鼠多发梗塞性痴呆(MID)模型;造模成功后分为模型组、针刺组和非穴组,并设正常组和假手术组作对照。针刺组在造模成功后第8天开始治疗,治疗后,采用放免法检测各组大鼠海马cAMP、cGMP含量。结果:与正常组比较,模型组海马cAMP含量明显下降(P0.01)。与模型组比较,针刺组cAMP含量升高(P0.01),接近正常组水平。与正常组比较,模型组cGMP含量未见明显异常变化,针刺组亦未见明显调节作用。结论:针刺足三里穴可以通过调节cAMP含量,介导血管平滑肌的松弛反应,改善脑血流供应。

Effect of acupuncture on hippocampal cAMP and cGMP contents in rats with multi-infarct dementia

Objective: To observe the changes of second messenger cAMP and cGMP contents due to acupuncture at the point ST36 on multi-infarct dementia rats.Method: The multi-infarct dementia model was made by injection of emboli into the internal carotid artery.The dementia rats were randomly divided into 3 groups: model group,acupuncture group and non-acupoint acupuncture group.A normal group and a sham operation group were set up as contrast.After treatment,the hippocampus cAMP and cGMP contents of each group by radioimmunoassay were detected.Result: Compared with normal group,cAMP in the hippocampus of the model group significantly decreased(P<0.01).Compared with model group,cAMP content in the hippocampus of the acupuncture group significantly increased(P<0.01),close to normal group level.Compared with normal group,cGMP content in the hippocampus of model group has the obvious changes;acupuncture group has not obvious regulatory role.Conclusion: Acupuncture at the point ST36 can mediated vascular smooth muscle relaxation response by adjusting the cAMP content,consequently improving blood supply to the brain.