| 钛种植体表面固定生物素的实验研究 |
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| 引用本文： | 杜明亮,钱海燕,吴明月,李全利.钛种植体表面固定生物素的实验研究[J].安徽医科大学学报,2015(4). |
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| 作者姓名： | 杜明亮 钱海燕 吴明月 李全利 |
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| 作者单位： | 安徽医科大学附属口腔医院，安徽医科大学口腔医学院，安徽省口腔疾病研究中心实验室，合肥 230032;安徽医科大学附属口腔医院，安徽医科大学口腔医学院，安徽省口腔疾病研究中心实验室，合肥 230032;安徽医科大学附属口腔医院，安徽医科大学口腔医学院，安徽省口腔疾病研究中心实验室，合肥 230032;安徽医科大学附属口腔医院，安徽医科大学口腔医学院，安徽省口腔疾病研究中心实验室，合肥 230032 |
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| 摘 要： | 目的：利用生物素-亲和素系统，将生物素共价键合在钛基材表面，为钛种植体表面接枝生物活性分子提供新的实验方法。方法使用3-氨丙基膦酸( APP)对钛基材表面进行酸蚀处理并使其获得游离氨基，由碳二亚胺( EDC )介导，通过与羧基反应形成酰胺键以实现生物素-亲和素系统在钛种植体表面的固定。结果通过环境扫描电镜、傅立叶变换红外光谱、X射线光电子能谱、免疫荧光等检测方法对样品进行表征，证实钛基材表面已成功接枝生物素。结论采用生物素-亲和素系统，可以在氨基化的钛基材表面成功接枝生物素，为钛种植体表面生物化修饰的研究提供新的方法和思路。
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| 关 键 词： | 钛种植体 3-氨丙基膦酸 生物素-亲和素系统 共价键合 |
The experimental study of immobilization of biotin on the titanium implant surface |
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| Abstract: | Objective To covalently immobilize biotin on the surface of the titanium substrate by biotin-avidin technology. Eventually, the study provides new experimental methods for titanium implant surface grafting bioactive molecules. Methods The surface of the titanium substrate was treated by 3-aminopropylphosphonic acid(APP), and enabled them to obtain free amino groups, which reacted with carboxyl groups to form amide bond to immobilize the biotin-avidin system on the surface of titanium implant by carbodiimide( EDC)-mediated. Results The samples were characterized by environmental scanning electron microscopy, fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and immunofluorescence, which demonstrated that biotin was successfully bonded to the titanium surface. Conclusion The biotin can be successfully immobilized on the surface of titanium implant with grafted amino groups through biotin-avidin technology. This study develops a new research approach and experimen-tal foundation of the biolization modification on the surface of titanium implant. |
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| Keywords: | titanium implant 3-aminopropylphosphonic acid biotin-avidin system covalently bonding |
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