| RANKL联合M-CSF体外诱导人外周血单个核细胞成为破骨样细胞的研究 |
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| 引用本文： | 祝捷,董崇周,胡圆圆,马尊良,陈超,邢学农,叶山东,王明丽,王晓秋,胡闻,李明,付圣权.RANKL联合M-CSF体外诱导人外周血单个核细胞成为破骨样细胞的研究[J].安徽医科大学学报,2010,45(5):618-620,703. |
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| 作者姓名： | 祝捷 董崇周 胡圆圆 马尊良 陈超 邢学农 叶山东 王明丽 王晓秋 胡闻 李明 付圣权 |
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| 作者单位： | 安徽医科大学附属省立医院内分泌科,合肥,230001;安徽医科大学微生物学教研室,合肥,230032;安徽医科大学附属省立医院病理科,合肥,230001;中国科学技术大学理化科学实验中心,合肥,230026 |
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| 摘 要： | 目的 体外诱导人外周血单个核细胞成为破骨样细胞.方法 从人外周血以密度梯度离心法分离出单个核细胞贴壁培养后分为3组.A组:使用核因子NF-κB受体激活剂配体(RANKL)联合巨噬细胞集落刺激因子(M-CSF)进行诱导;B组:仅使用M-CSF进行诱导;C组:仅使用RANKL进行诱导.14 d后使用抗酒石酸酸性磷酸酶(TRAP)染色法以及扫描电镜观察骨片以鉴定细胞是否具有破骨细胞的特征.结果 A组14 d时出现较多的多核TRAP染色阳性的细胞,数量为(476±47)个,较B组(54±18)个明显增加(P<0.05),C组不能诱导出TRAP染色阳性细胞.A组诱导生成的细胞具有在骨片上形成骨吸收陷窝的能力,B组骨片上均没有发现明显的骨吸收陷窝.结论 从人外周血单个核细胞中可以诱导出破骨样细胞,RANKL和M-CSF是诱导过程中所需要的两个重要因子.
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| 关 键 词： | 破骨细胞 体外研究 |
Induction of osteoclast-like cells from human peripheral blood mononuclear cells by RANKL and M-CSF in vitro |
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| Zhu Jie,Dong Chongzhou,Hu Yuanyuan,et al.Induction of osteoclast-like cells from human peripheral blood mononuclear cells by RANKL and M-CSF in vitro[J].Acta Universitis Medicinalis Anhui,2010,45(5):618-620,703. |
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| Authors: | Zhu Jie Dong Chongzhou Hu Yuanyuan |
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| Affiliation: | Zhu Jie,Dong Chongzhou,Hu Yuanyuan,et al (Dept of Endocrinology,The Affiliated Provincial Hospital of Anhui Medical University,Hefei 230001) |
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| Abstract: | Objective To induce osteoclast-like cells(OLCs) from human peripheral blood mononuclear cells(PBMCs) in vitro. Methods Mononuclear cells were isolated from human peripheral blood by density gradient centrifugation,then were divided into three groups: group A: cultured with medium containing RANKL and M-CSF; group B: cultured with medium containing M-CSF; group C: cultured with medium containing RANKL. OLCs were identified by the tartrate-resistant acid phosphatase(TRAP) staining and scanning electron micros... |
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| Keywords: | osteoclasts in vitro |
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