响应面法优化竹叶香豆素提取工艺

为优化竹叶香豆素的提取工艺。在单因素试验基础上,进行四因素三水平的中心组合试验,用软件Design-Expert 8.0.1拟合响应值与影响因素的关系得到模型方程,分析模型方程得出提取香豆素最佳工艺:乙醇体积分数43.75%、提取温度80℃、提取时间50 min、液料比20︰1(mL·g^-1)。经验证,竹叶香豆素得率的试验值1.785 mg/g和预测值1.870 mg/g的相对误差为4.54%,说明模型可靠性高,运用响应面法优化竹叶香豆素的提取工艺条件的方法具有可行性。

Optimization of Extraction Process of Coumarin from Bamboo Leaves by Response Surface Methodology

In order to optimize the extraction process of bamboo leaf coumarin,on the basis of the single factor experiment,a four-factor and three-level central combination test was carried out,the relationship between the response value and the influencing factors was obtained by software Design-Expert 8.0.1 to obtain the model equation,and the model equation was analyzed to obtain the extracted bamboo leaves.The best process was ethanol content 43.75%,extraction temperature 80℃,extraction time 50 min,liquid to material ratio 20︰1(mL·g^-1).It was verified that the relative error of the experimental value of 1.785 mg/g and the predicted value of 1.870 mg/g of bamboo coumarin was 4.54%,indicating that the model had high reliability,and the extraction process of bamboo coumarin was optimized by response surface methodology.The method of condition was feasible.