| SARS病毒对温度耐受性的实验研究 |
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| 引用本文： | 鲍作义,刘永健,刘思扬,庄道民,李敬云.SARS病毒对温度耐受性的实验研究[J].中国消毒学杂志,2003,20(3):161-162. |
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| 作者姓名： | 鲍作义 刘永健 刘思扬 庄道民 李敬云 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 摘 要： | 为观察SARS病毒在冷藏和自然温度下的稳定性以及灭活SARS病毒的有效温度,将SARS病毒培养的上清液(10~6TCID_(50))分装到10ml离心管中,放置在4℃冰箱、室温(24.5℃)、37℃、56℃和70℃水浴中,间隔一定时间以后取样0.5ml,接种Vero-E6细胞。结果,SARS病毒在冷藏和室温条件下十分稳定,冷藏(4℃)放置10d,滴度只下降大约2个log,室温放置5d,滴度下降大约4个log,仍然保持较高的感染性。加热时SARS病毒的滴度迅速下降,56℃加热30min、70℃加热15min检测不出有活病毒。结论,SARS病毒在冷藏和自然温度下十分稳定,加热是灭活SARS病毒简单有效的方法。
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| 关 键 词： | SARS病毒 温度 加热 |
| 文章编号： | 1001-7658(2003)09-0161-03 |
| 修稿时间： | 2003年5月8日 |
ENDURANCE OF SARS VIRUS TO DIFFERENT TEMPERATURES |
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| Bao Zuoyi Liu Yongjian Liu Siyang Zhuang Daomin Li Jingyun.ENDURANCE OF SARS VIRUS TO DIFFERENT TEMPERATURES[J].Chinese Journal of Disinfection,2003,20(3):161-162. |
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| Authors: | Bao Zuoyi Liu Yongjian Liu Siyang Zhuang Daomin Li Jingyun |
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| Abstract: | Objective: To evaluate the sensitivity of SARS virus to different temperatures. Methods: The supernatant of cell culture of the virus was put in 4℃ refrigerator, room temperature (24.5 ℃), waterbath of 37℃ , 56℃ and 70℃ . Samples were taken at definite time points and inoculated in Vero-E6 cells. The cytopathic effect (CPE) was observed and recorded after 4 days. Results: SARS virus was very stable at 4℃ and room temperature. It was infectious at least for 10 days at 4℃. Its titer decreased from 7.5 TCID50 to 3.2 TCID50 within 5 days at room temperature. The virus was sensitive to heating. After staying at 37℃ for 4 days, 56℃ for 30 minutes and 70℃ for 15 minutes, the virus lost its infectivity. Conclusions: SARS virus is very sensitive to high temperature. Heating is an effective method to inactive the virus. |
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| Keywords: | SARS virus temperature heating |
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