| 重组人肝脏增生因子克隆表达纯化及生物活性的探讨 |
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| 引用本文： | 曾晓波,苏先狮.重组人肝脏增生因子克隆表达纯化及生物活性的探讨[J].中国基层医药,2008,15(11). |
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| 作者姓名： | 曾晓波 苏先狮 |
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| 作者单位： | 中南大学湘雅二医院肝病研究所,湖南省长沙,410011 |
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| 摘 要： | 目的 构建人肝脏再生增强因子(hALR)原核表达载体,为基因工程大量制备hALR提供基础.方法 构建hALR表达载体pGEX-3X-hALR,诱导表达GST-hALR,用GST Agaroee柱亲和层析,再用Xa因子切除GST得到hAIR蛋白;进一步用MTT法及动物实验检测hALR活性.结果 成功地构建了hALR表达载体pGEX-3X-hALR,并纯化出hALR蛋白;体外细胞实验及动物实验结果与文献报道相反.结论
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| 关 键 词： | 人肝脏增生因子 克隆 分子 蛋白纯化 活性检测 |
The cloning,expression and pruification of recombinant |
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| ZENG Xia-obo,SU Xian-shi.The cloning,expression and pruification of recombinant[J].Chinese Journal of Primary Medicine and Pharmacy,2008,15(11). |
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| Authors: | ZENG Xia-obo SU Xian-shi |
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| Abstract: | Objective To provide a technique of preparation recombinant hALR by gene engineering in larger scale.Methods Construct hALR(human augmenter of liver regeneration) protein expression vector pGEX-3X-hALR,induce the expreesion of hALR,and pruified the protein by affinity chromatography.Results Succeed in constructing the vector pGEX-3X-hALR and obtained the recombinant hALR protein.Conclusion The mothed could be used for preparation recombinant hALR by gene engineering in larger scale. |
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| Keywords: | Human augmenter of Iiver regeneration Cloning molecular Expression Purification |
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