γ-干扰素释放反应与PPD皮试在肾结核诊断应用比较

目的：采用结核分枝杆菌特异性IFN-γ免疫酶联斑点法（Elispot）与传统的结核菌素（PPD）皮试对肾结核患者及健康人群结核分枝杆菌感染现状进行检查，并比较两种方法的差异。方法：采用自主研制的Elispot试剂盒和PPD皮试，对14例肾结核患者及133例健康志愿者进行平行检测。结果：14例肾结核患者检测中，13例PPD阳性，12例Elispot阳性，差异无统计学意义。133例志愿者中86例PPD阳性，22例Elispot阳性，差异有统计学意义。结论：Elispot试剂盒在健康人群中检测阳性率远低于PPD皮试，而在肾结核的检测中差异无统计学意义。Elispot在区分肾结核患者与健康人方面价值优于PPD皮试。在肾结核的诊断有较好的发展前景。

The Initial Research of Using ELISPOT with PPD in the Diagnosis of Renal Tuberculosis

Objective:To compare Mycobacterium Tuberculosis specific IFN-γElispot assays and PPD skin test in patients with renal tuberculosis and healthy volunteers.Methods;14 renal tuberculosis and 133 healthy volunteers of two colleges in Shenzhen were accepted the in-house Elispot test and PPD skin test.Results:Of 14 cases of renal tuberculosis,13 cases were positive in response to PPD skin test,and 12 cases were positive in response to in-house Elispot test.The two tests have no significant difference.Of 133 cases of healthy volunteers.86 cases were positive to PPD,and 22 were positive to Elispot.There was significant different between the two tests.Conclusions:The proportion of in-house Elispot was far lower than the PPD skin test in volunteers while they have no significant different in renal tuberculosis patients.Elispot was better than the PPD skin test for telling renal tuberculosis patients from healthy people and it would have a good prospects in early diagnosis of renal tuberculosis.