KLF6蛋白在散发性结直肠癌组织中的表达及其与p21蛋白的关系

目的 分析Krupple样转录因子6(KLF6)蛋白在结直肠癌中的表达及其与p21蛋白的关系,探讨KLF6蛋白及p21蛋白在结直肠癌中发生发展中的作用.方法 应用免疫组织化学SP法检测KLF6蛋白及p21蛋白在结直肠癌、结直肠腺瘤及正常结直肠组织中的表达,分析KLF6蛋白及p21蛋白与结直肠癌各种临床病理参数的关系,探讨结直肠癌中KLF6与p21蛋白表达的关系.结果 KLF6蛋白及p21蛋白在正常结直肠组织、结直肠腺瘤到结直肠癌组织中表达逐渐下调(85.7%、76.5%、43.6%),三者比较差异有统计学意义(x2=25.20,P＜0.01);KLF6蛋白表达水平与组织学类型无关(x2=0.13,P＞0.05),与分化程度(x2=4.04)、淋巴结转移(x2=6.79)、TNM分期(x2=6.16)有关(P＜0.05).KLF6与p21蛋白在结直肠癌组织中的表达呈正相关(r=0.74,P＜0.05).结论 KLF6表达下降,可能参与了结直肠癌的发生发展;KLF6基因可能是结直肠癌的一个重要抑癌基因并参与结直肠癌细胞凋亡的凋节.KLF6与p21蛋白表达呈正相关,p21可能是KLF6基因的效应蛋白.

Expression of krupple like factor 6 protein in colorectal cancer tissue and its relationship with p21 protein

Objective To study krupple like factor 6 (KLF6) protein expression in colorectal cancer (CRC) tissue, evaluate its possible role in the tumorigenesis and progression of CRC, and investigate its relationship with the expression of p21 protein. Methods The expression of KLF6 protein and p21 protein in CRC tissue, colorectal adenoma tissue, and normal colorectal tissues was detected by the immunohistochemistry method. The possible relationship between two protein' s expression and clinicopathological parameters was analyzed with SPSS software for Windows. Results The expression of KLF6 protein was gradually down-regulated from normal colorectal tissues to colorectal adenoma tissue to CRC tissue (x2= 25.20, P ＜ 0.01 ). Spearman rank correlation analysis showed a strong inverse correlation between the expression of KLF6 and p21 protein (r=0.74,P＜0.05). Conclusion KLF6 plays a considerable inhibitory action in genesis and development of CRC. Abnormal expression of KLF6 might have inhibitory effect on invasion and metastasis of CRC and act as an effective and objective marker to indicate the pathobiological behavior of CRC. The expression of KLF6 was positively correlated to the p21. Furthermore p21 may be an effective protein of KLF6 gene.