乌贼墨对环磷酰胺致小鼠脾脏氧化性损伤的保护效应

探讨乌贼墨提取物对环磷酰胺致小鼠脾脏氧化性损伤拮抗作用。40 只昆明小鼠，随机分成4 组，雌雄各半，各组分别灌服生理盐水或不同剂量的乌贼墨提取物及腹腔注射生理盐水或环磷酰胺。观察各组小鼠脾脏器官指数，脾脏中MDA、SOD、CAT、GSH-Px 含量，以及外周血白细胞数量。与对照组比较，环磷酰胺能显著的降低正常小鼠的脾脏指数(p ＜ 0.05)，能极显著的降低正常小鼠脾脏组织中SOD、CAT、GSH-Px 活力(p ＜ 0.01)，显著的提高正常小鼠脾脏组织中MDA 含量(p ＜ 0.05)，也能极其显著的降低正常小鼠外周血白细胞数量(p ＜ 0.01)。与模型组比较，适当浓度的乌贼墨提取物对环磷酰胺所致各项指标的变化均有不同程度的改善作用(p ＜ 0.05 或p ＜0.01)，结果表明，较高浓度的乌贼墨提取物的改善作用反而不是很明显，相对于低剂量组，在一定程度上起到抑制作用。乌贼墨提取物对环磷酰胺所致的小鼠脾脏组织氧化性损伤进而导致的免疫抑制具有一定的改善和治疗作用，是一种有效的而且很有潜力的天然药物资源。

Protective Effect of Sepia Ink Extract against Cyclophosphamide-induced Oxidative Damage in Mice Spleen

A total of 40 Kunming mice were randomly divided into 4 groups (group A, group B, group C and group D), with half male and half female. Group A as the control was subject to oral administration and then intraperitoneal injection of normal saline. Group B as the oxidative damage model, group C and group D all were subject to intraperitoneal injection of cyclophosphamide and then oral administration of normal saline, and low dose and high dose of squid ink extract, respectively. The organ index of spleen, content of MDA and the activities of SOD, CAT and GSH-Px in spleen tissue were determined, and the quantity of leukocytes in peripheral blood was counted. Compared to the control group, cyclophosphamide was able to decrease the organ indexes of spleen (p ＜ 0.05), as well as the SOD, CAT and GSH-PX activities (p ＜ 0.01) and increase MDA level in spleen tissue. Compared to the oxidative damage model group, high and low doses of squid ink extract could improve the above oxidative damage symptoms at different degrees, but the therapeutic effect of the former was not more apparent than that of the latter was. In conclusion, sepia ink extract at the suitable concentration is able to alleviate the in vivo immunosuppression induced by cyclophosphamide in mice.