高效液相色谱法对绿茶中茶多酚含量的测定

采用高效液相色谱法测定绿茶中茶多酚的含量。在色谱柱为Kromasil C18、流动相为乙酸:甲醇:N,N- 二甲基甲酰胺:水=1:1:40:58(V/V) 、色谱柱温30℃、进样量10μL、检测器为紫外检测器、检测波长280.0nm 的色谱条件下对绿茶中茶多酚含量进行测定。结果表明：回归方程为Y=1 × 10-7X － 0.0036(r=0.9995)，方法变异系数小于3.04%，回收率为99.16%～100.60%；6 种绿茶样品茶多酚含量为62.07～110.86mg/g，说明产地不同，绿茶中茶多酚的含量相差较大。本方法简便、准确，适合绿茶中茶多酚含量的分析。

HPLC Determination of Polyphenols in Green Tea

The polyphenol contents in green tea were determined by high performance liquid chromatography method, using the mobile phase consisting of acetic acid, methyl alcohol, N,N- dimethylformamide and warter (1:1:40:58, V/V). The column temperature was 30 ℃, and the detection wavelength was 280.0 nm. The regression equation obtained was Y=1×10-7X－0.0036 (r =0.9995). The coefficient of variation was less than 3.04%, and the recovery was 99.16%－100.60%. The result indicates that the proposed method is simple , accurate and feasible for determination of polyphenols in the green teas.